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Regular Article |
and ß in the Reproductive Tractsof Adult Male Dogs and Cats1
a Department of Veterinary Biosciences, University of Illinois, Urbana, Illinois 61802
b MRC Human Reproductive Sciences Unit, Edinburgh EH3 9ET, United Kingdom
Expression of estrogen receptors (ERs) in the reproductive tracts of adult male dogs and cats has not been reported. In the present study, ER
and ERß were localized by immunohistochemistry using ER-specific antibodies. ER was found in interstitial cells and peritubular myoid cells in the dog testis, but only in interstitial cells of the cat. In rete testis of the dog, epithelial cells were positive for ER staining, but in the cat, rete testis epithelium was only weakly positive. In efferent ductules of the dog, both ciliated and nonciliated cells stained intensely positive. In the cat, ciliated epithelial cells were less stained than nonciliated epithelial cells. Epithelial cells in dog epididymis and vas deferens were negative for ER. In the cat, except for the initial region of caput epididymis, ER staining was positive in the epithelial cells of epididymis and vas deferens. Multiple cell types of dog and cat testes stained positive for ERß. In rete testis and efferent ductules, epithelial cells were weakly positive for ERß. Most epithelial cells of the epididymis and vas deferens exhibited a strong positive staining in both species. In addition, double staining was used to demonstrate colocalization of both ER and ERß in efferent ductules of both species. The specificity of antibodies was demonstrated by Western blot analysis. This study reveals a differential localization of ER and ERß in male dog and cat reproductive tracts, demonstrating more intensive expression of ERß than ER. However, as in other species, the efferent ductules remained the region of highest concentration of ER.
1 R.A.H. was supported by the Kenneth A. Scott Charitable Trust and by grant HD35126 from the National Institutes of Health.
2 Correspondence: Rex A. Hess, Department of Veterinary Biosciences, University of Illinois, 2001 S. Lincoln, Urbana, IL 61802. FAX: 217 244 1652; r-hess{at}uiuc.edu
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