| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Department of Biology, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan
b Laboratory of Molecular Genetics, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512, Japan
Two distinct cDNAs encoding ß subunits of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were cloned from the cDNA library constructed for the pituitary of the red-bellied newt, Cynops pyrrhogaster, and sequenced. The newt FSHß and LHß cDNAs encode polypeptides of 129 and 131 amino acids, including signal peptides of 20 and 19 amino acids, respectively. The number and position of cysteine and N-glycosylation in each of the ß subunits of FSH and LH, which are considered essential for assembly of the 
subunit, are well conserved between the newt and other tetrapods. The high homology (41.6%) between the ß subunits of newt FSH and LH imply less specificity of FSH and LH in gonadal function. One cDNA encoding the common polypeptide chain subunit of FSH and LH was also isolated from the newt pituitary gland. The mRNAs of FSHß, LHß, and the subunit were expressed only in the pituitary gland among various newt tissues. Double-staining with in situ hybridization and immunohistochemistry revealed coexpression of FSHß and LHß in the same newt pituitary cells. Ovariectomy induced a significant increase in FSHß mRNA levels, but there was no significant change in LHß or subunit mRNA levels compared with those in control animals. Taken together, these data suggest that two kinds of gonadotropins, namely FSH and LH, are expressed in the same gonadotropin-producing cells in the pars distalis of the newt as well as in other tetrapods and that the expression of FSHß is negatively regulated by the ovaries.
1 This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan to S.T.
2 Correspondence: Shigeyasu Tanaka, Department of Biology, Faculty of Science, Shizuoka University, Ohya 836, Shizuoka 422-8529, Japan. FAX: 81 54 238 0986; sbstana{at}ipc.shizuoka.ac.jp
This article has been cited by other articles:
|
|
 |
|
  U. W Khan and U. Rai Differential effects of histamine on Leydig cell and testicular macrophage activities in wall lizards: precise role of H1/H2 receptor subtypes J. Endocrinol., August 1, 2007; 194(2): 441 - 448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yaoi, T. Onda, Y. Hidaka, S. Yajima, M. Suzuki, and S. Tanaka Developmental Expression of Otoconin-22 in the Bullfrog Endolymphatic Sac and Inner Ear J. Histochem. Cytochem., May 1, 2004; 52(5): 663 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Querat, Y. Arai, A. Henry, Y. Akama, T. J. Longhurst, and J. M.P. Joss Pituitary Glycoprotein Hormone {beta} Subunits in the Australian Lungfish and Estimation of the Relative Evolution Rate of These Subunits Within Vertebrates Biol Reprod, February 1, 2004; 70(2): 356 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yaoi, M. Suzuki, H. Tomura, S. Kurabuchi, Y. Sasayama, and S. Tanaka Expression and Localization of Prohormone Convertase PC1 in the Calcitonin-producing Cells of the Bullfrog Ultimobranchial Gland J. Histochem. Cytochem., November 1, 2003; 51(11): 1459 - 1466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yaoi, M. Suzuki, H. Tomura, Y. Sasayama, S. Kikuyama, and S. Tanaka Molecular Cloning of Otoconin-22 Complementary Deoxyribonucleic Acid in the Bullfrog Endolymphatic Sac: Effect of Calcitonin on Otoconin-22 Messenger Ribonucleic Acid Levels Endocrinology, August 1, 2003; 144(8): 3287 - 3296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.F. Vischer, A.C.C. Teves, J.C.M. Ackermans, W. van Dijk, R.W. Schulz, and J. Bogerd Cloning and Spatiotemporal Expression of the Follicle-Stimulating Hormone {beta} Subunit Complementary DNA in the African Catfish (Clarias gariepinus) Biol Reprod, April 1, 2003; 68(4): 1324 - 1332. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
