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Germ Line Stem Cell Competition in Postnatal Mouse Testes¹

^a Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Niche is believed to affect stem cell behavior. In self-renewing systems for which functional transplantation assays are available, it has long been assumed that stem cells are fixed in the niche and that ablative treatments to remove endogenous stem cells are required for successful donor engraftment. Our results demonstrate that enriched populations of donor stem cells can produce long-lasting spermatogenic colonies in testes of immature and mature, nonablated mice, albeit at a lower frequency than in ablated mice. Colonization of nonablated recipient testes by neonate, pup, and cryptorchid adult donor spermatogonial stem cells demonstrates that competition for niche begins soon after birth and that endogenous stem cells influence the degree and pattern of donor cell colonization. Thus, a dynamic relationship between stem cell and niche exists in the testis, as has been suggested for hematopoiesis. Therefore, similar competitive properties of donor stem cells may be characteristic of all self-renewing systems.

First decision: 26 November 2001.

¹ T.S. was supported by the Japan Society for Promotion of Science. Microscopic sections were produced in the Institute for Human Gene Therapy, Cellular Morphology Core (5-P30-DK-47747-07). Financial support for the research was from the NIH (NICHD 36504), Commonwealth and General Assembly of Pennsylvania, and the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation.

² Correspondence: Ralph L. Brinster, School of Veterinary Medicine, University of Pennsylvania, 3850 Baltimore Avenue, Philadelphia, PA 19104. FAX: 215 898 0667

³ Current address: Department of Medical Chemistry, Kyoto University, Yoshida-Konoe, Sakyo-ku, Kyoto 606-0667, Japan

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