| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Magee-Womens Research Institute and the Department of Obstetrics and Gynecology and Reproductive Biology of the University of Pittsburgh, Pittsburgh, Pennsylvania 15213
Paracrine and autocrine growth factors can affect many different aspects of ovarian follicle development. Many members of the transforming growth factor ß (TGFß) family of growth factors and their receptors are expressed in developing follicles. However, the presence and function of the family of the TGFß signaling molecules known as Smads have not been evaluated during follicle development. We have demonstrated that two Smad family members that function as mediators for both activin and TGFß are expressed in granulosa cells of preantral follicles but not in large antral follicles. Smad2 expression, but not Smad3 expression, returns in luteal cells. Both Smad2 and Smad3 are translocated to the nucleus of granulosa cells in response to treatment with either TGFß or activin. However, Smad2 is more responsive to activin stimulation, and Smad3 is more responsive to TGFß stimulation. Stage-specific expression and differing ligand sensitivity of signaling molecules may work together to allow different effects of TGFß family ligands using the same signaling pathways over the course of follicular development.
1 This work was supported by Magee-Womens Research Institute Postdoctoral Fellowship (J.X.), the American Society of Reproductive Medicine/Ortho MacNeil Award in Reproductive Medicine (E.A.M.), and Women's Reproductive Health Research Center Program sponsored by the National Institute of Child Health and Human Development (E.A.M.).
2 Correspondence: Elizabeth A. McGee, MWRI, 204 Craft Ave., Pittsburgh, PA 15213. FAX: 412 641 5290; rsieam{at}mail.magee.edu
This article has been cited by other articles:
|
|
 |
|
  Q. Li, S. A. Pangas, C. J. Jorgez, J. M. Graff, M. Weinstein, and M. M. Matzuk Redundant Roles of SMAD2 and SMAD3 in Ovarian Granulosa Cells In Vivo Mol. Cell. Biol., December 1, 2008; 28(23): 7001 - 7011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Li, J. M. Graff, A. E. O'Connor, K. L. Loveland, and M. M. Matzuk SMAD3 Regulates Gonadal Tumorigenesis Mol. Endocrinol., October 1, 2007; 21(10): 2472 - 2486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, J.-Y. Jiang, C. Zhu, C. Peng, and B. K. Tsang Role and Regulation of Nodal/Activin Receptor-Like Kinase 7 Signaling Pathway in the Control of Ovarian Follicular Atresia Mol. Endocrinol., October 1, 2006; 20(10): 2469 - 2482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mazerbourg and A. J.W. Hsueh Genomic analyses facilitate identification of receptors and signalling pathways for growth differentiation factor 9 and related orphan bone morphogenetic protein/growth differentiation factor ligands Hum. Reprod. Update, July 1, 2006; 12(4): 373 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Yang and S. K. Roy Transforming Growth Factor B1 Stimulated DNA Synthesis in the Granulosa Cells of Preantral Follicles: Negative Interaction with Epidermal Growth Factor Biol Reprod, July 1, 2006; 75(1): 140 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Park, E. T. Maizels, Z. J. Feiger, H. Alam, C. A. Peters, T. K. Woodruff, T. G. Unterman, E. J. Lee, J. L. Jameson, and M. Hunzicker-Dunn Induction of Cyclin D2 in Rat Granulosa Cells Requires FSH-dependent Relief from FOXO1 Repression Coupled with Positive Signals from Smad J. Biol. Chem., March 11, 2005; 280(10): 9135 - 9148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.L. Juengel and K.P. McNatty The role of proteins of the transforming growth factor-{beta} superfamily in the intraovarian regulation of follicular development Hum. Reprod. Update, March 1, 2005; 11(2): 144 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Pierre, C Pisselet, J Dupont, B Mandon-Pepin, D Monniaux, P Monget, and S Fabre Molecular basis of bone morphogenetic protein-4 inhibitory action on progesterone secretion by ovine granulosa cells J. Mol. Endocrinol., December 1, 2004; 33(3): 805 - 817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Tomic, K. P. Miller, H. A. Kenny, T. K. Woodruff, P. Hoyer, and J. A. Flaws Ovarian Follicle Development Requires Smad3 Mol. Endocrinol., September 1, 2004; 18(9): 2224 - 2240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kalinina, A. Agrotis, Y. Antropova, O. Ilyinskaya, V. Smirnov, E. Tararak, and A. Bobik Smad Expression in Human Atherosclerotic Lesions: Evidence for Impaired TGF-{beta}/Smad Signaling in Smooth Muscle Cells of Fibrofatty Lesions Arterioscler. Thromb. Vasc. Biol., August 1, 2004; 24(8): 1391 - 1396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mazerbourg, C. Klein, J. Roh, N. Kaivo-Oja, D. G. Mottershead, O. Korchynskyi, O. Ritvos, and A. J. W. Hsueh Growth Differentiation Factor-9 Signaling Is Mediated by the Type I Receptor, Activin Receptor-Like Kinase 5 Mol. Endocrinol., March 1, 2004; 18(3): 653 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Gueripel, M. Benahmed, and A. Gougeon Sequential Gonadotropin Treatment of Immature Mice Leads to Amplification of Transforming Growth Factor {beta} Action, Via Upregulation of Receptor-Type 1, Smad 2 and 4, and Downregulation of Smad 6 Biol Reprod, March 1, 2004; 70(3): 640 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xu, A. R. Beyer, W. H. Walker, and E. A. McGee Developmental and Stage-Specific Expression of Smad2 and Smad3 in Rat Testis J Androl, March 1, 2003; 24(2): 192 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kaivo-Oja, J. Bondestam, M. Kamarainen, J. Koskimies, U. Vitt, M. Cranfield, K. Vuojolainen, J. P. Kallio, V. M. Olkkonen, M. Hayashi, et al. Growth Differentiation Factor-9 Induces Smad2 Activation and Inhibin B Production in Cultured Human Granulosa-Luteal Cells J. Clin. Endocrinol. Metab., February 1, 2003; 88(2): 755 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Moore, F. Otsuka, and S. Shimasaki Molecular Basis of Bone Morphogenetic Protein-15 Signaling in Granulosa Cells J. Biol. Chem., January 3, 2003; 278(1): 304 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Norwitz, S. Xu, J. Xu, L. B. Spiryda, J. S. Park, K.-H. Jeong, E. A. McGee, and U. B. Kaiser Direct Binding of AP-1 (Fos/Jun) Proteins to a SMAD Binding Element Facilitates Both Gonadotropin-releasing Hormone (GnRH)- and Activin-mediated Transcriptional Activation of the Mouse GnRH Receptor Gene J. Biol. Chem., September 27, 2002; 277(40): 37469 - 37478. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
