| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Embryo Transplantation Laboratory, Snow Brand Milk Products Co. Ltd., 119 Uenae, Tomakomai, Hokkaido 059-1365, Japan
b Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
To test the hypothesis that oocytes require time to acquire developmental competence during meiotic arrest, we investigated the effects of butyrolactone I (BL I), a potent and specific inhibitor of cyclin-dependent kinase, on the developmental competence of bovine oocytes after in vitro fertilization (IVF) following release from meiotic arrest. In the present study, 4 culture conditions were used: addition of BSA or fetal bovine serum (FBS) under 2 oxygen tensions (5% vs. 20%) during meiotic arrest with 100-µM BL I. The developmental competence to the blastocyst stage was higher (P < 0.01) in oocytes that were arrested in FBS-supplemented medium under 5% O2 (37%) than in oocytes that were arrested under other conditions (5%–24%) or that matured directly following follicle aspiration (23%). The time course of nuclear maturation of BL I-treated oocytes was also examined. The results demonstrated that oocytes treated with BL I start germinal vesicle (GV) breakdown and reach the metaphase II stage 5.5–6.0 h earlier than nonarrested oocytes. The developmental rates to the blastocyst stage of BL I-treated oocytes matured for 15.5 and 21 h were higher (P < 0.05) than those of nontreated oocytes matured for 21 and 26.5 h, respectively. These results demonstrate that bovine immature oocytes, which were arrested at the GV stage with BL I in FBS-supplemented medium under low oxygen tension, acquire higher developmental competence during meiotic arrest.
1 Part of this work was supported by a grant from the Japan Society for the Promotion of Science (JPS-RFTF 97L00905 to N.M.).
2 Correspondence. FAX: 81 75 753 6329; naojiro{at}kais.kyoto-u.ac.jp
3 Current address: Research & Development Center for Dairy Farming, Chuo-ku, Sapporo 060-0003, Japan
This article has been cited by other articles:
|
|
 |
|
  D. Nogueira, R. Ron-El, S. Friedler, M. Schachter, A. Raziel, R. Cortvrindt, and J. Smitz Meiotic Arrest In Vitro by Phosphodiesterase 3-Inhibitor Enhances Maturation Capacity of Human Oocytes and Allows Subsequent Embryonic Development Biol Reprod, January 1, 2006; 74(1): 177 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hinrichs, Y.H. Choi, L.B. Love, D.D. Varner, C.C. Love, and B.E. Walckenaer Chromatin Configuration Within the Germinal Vesicle of Horse Oocytes: Changes Post Mortem and Relationship to Meiotic and Developmental Competence Biol Reprod, May 1, 2005; 72(5): 1142 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.E. Thomas, J.G. Thompson, D.T. Armstrong, and R.B. Gilchrist Effect of Specific Phosphodiesterase Isoenzyme Inhibitors During In Vitro Maturation of Bovine Oocytes on Meiotic and Developmental Capacity Biol Reprod, October 1, 2004; 71(4): 1142 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nogueira, R. Cortvrindt, D.G. De Matos, L. Vanhoutte, and J. Smitz Effect of Phosphodiesterase Type 3 Inhibitor on Developmental Competence of Immature Mouse Oocytes In Vitro Biol Reprod, December 1, 2003; 69(6): 2045 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nogueira, C. Albano, T. Adriaenssens, R. Cortvrindt, C. Bourgain, P. Devroey, and J. Smitz Human Oocytes Reversibly Arrested in Prophase I by Phosphodiesterase Type 3 Inhibitor In Vitro Biol Reprod, September 1, 2003; 69(3): 1042 - 1052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shimada, M. Nishibori, N. Isobe, N. Kawano, and T. Terada Luteinizing Hormone Receptor Formation in Cumulus Cells Surrounding Porcine Oocytes and Its Role During Meiotic Maturation of Porcine Oocytes Biol Reprod, April 1, 2003; 68(4): 1142 - 1149. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
