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Regular Article |
a Animal Developmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejeon 305-600, Korea
b National Livestock Research Institute, Chonan 330-800, Korea
c Department of Biotechnology, Taegu University, Kyungsan 712-714, Korea
Abortions of nuclear transfer (NT) embryos are mainly due to insufficient placentation. We hypothesized that the primary cause might be the aberrant allocations of two different cell lineages of the blastocyst stage embryos, the inner cell mass (ICM) and the trophectoderm (TE) cells. The potential for development of NT embryos to blastocysts was similar to that for in vitro fertilized (IVF) embryos. No difference in the total cell number was detected between NT and IVF blastocysts, but both types of embryos had fewer total cells than did in vivo-derived embryos (P < 0.05). The NT blastocysts showed a higher ratio of ICM:total cells than did IVF or in vivo-derived embryos (P < 0.05). Individual blastocysts were assigned to four subgroups (I: <20%, II: 20–40%, III: 40–60%, IV: >60%) according to the ratio of ICM:total cells. Most NT blastocysts were placed in groups III and IV, whereas most IVF and in vivo-derived blastocysts were distributed in group II. Our findings suggest that placental abnormalities or early fetal losses in the present cloning system may be due to aberrant allocations of NT embryos to the ICM and TE cells during early development.
1 This study was supported by grants NLM0050111 and HSC0130134 from the Ministry of Science and Technology, Seoul, Korea.
2 Correspondence: Yong-Mahn Han, Animal Developmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Daejeon 305-600, Korea. FAX: 82 42 860 4608; e-mail: ymhan@mail.kribb.re.kr
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