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Regular Article |
a Graduate School of Applied Biosciences
b Department of Bioresources, Hiroshima Prefectural University, Hiroshima 727-0023, Japan
c Institute for Biogenesis Research, University of Hawaii School of Medicine, Honolulu, Hawaii 96822
The golden hamster is the mammalian species in which intracytoplasmic sperm injection (ICSI) was first tried to produce fertilized oocytes. Thus far, however, there are no reports of full-term development of hamster oocytes fertilized by ICSI. Here we report the birth of hamster offspring following ICSI. Keys to success were 1) performing ICSI in a dark room with a small incandescent lamp and manipulating both oocytes and fertilized eggs under a microscope with a red light source and 2) injecting sperm heads without acrosomes. All oocytes injected with acrosome-intact sperm heads died within 3 h after injection, while those oocytes injected with acrosomeless sperm heads survived injection. Under illumination with red light in a dark room, the majority of the oocytes injected with acrosomeless sperm heads were fertilized normally (77%), cleaved (91%), and developed into morulae (49%). Of the 47 morulae transferred to five recipient females, nine (19%) developed to live offspring.
1 Correspondence: Toshitaka Horiuchi, Department of Bioresources, Hiroshima Prefectural University, Shoubara, Hiroshima 727-0023, Japan. FAX: 81 8247 4 1750; toshi{at}bio.hiroshima-pu.ac.jp
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