| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China
b Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, Missouri 65211
Polo-like kinases (Plks) are a family of serine/threonine protein kinases that have been activated through phosphorylation. The activity of these kinases has been shown to be required for regulating multiple stages of mitotic progression in somatic cells. In this experiment, the changes in Plk1 expression were detected in mouse oocytes through Western blotting. The subcellular localization of Plk1 during oocyte meiotic maturation, fertilization, and early cleavage as well as after antibody microinjection or microtubule assembly disturbance was studied by confocal microscopy. The quantity of Plk1 protein remained stable during meiotic maturation and decreased gradually after fertilization. Plk1 was localized to the spindle poles of both meiotic and mitotic spindles at the early M phase and then translocated to the middle region. At anaphase and telophase, Plk1 was concentrated at the midbody of cytoplasmic cleavages. Plk1 was concentrated between the male and female pronuclei after fertilization. Plk1 disappeared at the spindle region when microtubule formation was inhibited by colchicine or staurosporine, while it was concentrated as several dots in the cytoplasm after taxol treatment. Plk1 antibody injection decreased the germinal vesicle breakdown rate and distorted MI spindle organization. Our results indicate that Plk1 is a pivotal regulator of microtubule organization during mouse oocyte meiosis, fertilization, and cleavage and that its functions may be regulated by other kinases, such as staurosporine-sensitive kinases.
1 Supported by grants from the Special Funds for Major State Basic Research (973) Project (G1999055902) and Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-SW-303).
2 Correspondence. FAX: 8610 6256 5689; sunqy{at}panda.ioz.ac.cn
This article has been cited by other articles:
|
|
 |
|
  B. Xiong, S. Li, J.-S. Ai, S. Yin, Y.-C. OuYang, S.-C. Sun, D.-Y. Chen, and Q.-Y. Sun BRCA1 Is Required for Meiotic Spindle Assembly and Spindle Assembly Checkpoint Activation in Mouse Oocytes Biol Reprod, October 1, 2008; 79(4): 718 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-J. Huo, C.-G. Liang, L.-Z. Yu, Z.-S. Zhong, Z.-M. Yang, H.-Y. Fan, D.-Y. Chen, and Q.-Y. Sun Inducible nitric oxide synthase-derived nitric oxide regulates germinal vesicle breakdown and first polar body emission in the mouse oocyte Reproduction, April 1, 2005; 129(4): 403 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Manandhar, H. Schatten, and P. Sutovsky Centrosome Reduction During Gametogenesis and Its Significance Biol Reprod, January 1, 2005; 72(1): 2 - 13. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhang, M. Li, W. Ma, Y. Hou, Y.-H. Li, S.-W. Li, Q.-Y. Sun, and W.-H. Wang Localization of Mitotic Arrest Deficient 1 (MAD1) in Mouse Oocytes During the First Meiosis and Its Functions as a Spindle Checkpoint Protein Biol Reprod, January 1, 2005; 72(1): 58 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-J. Huo, H.-Y. Fan, C.-G. Liang, L.-Z. Yu, Z.-S. Zhong, D.-Y. Chen, and Q.-Y. Sun Regulation of Ubiquitin-Proteasome Pathway on Pig Oocyte Meiotic Maturation and Fertilization Biol Reprod, September 1, 2004; 71(3): 853 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-J. Yao, Z.-S. Zhong, L.-S. Zhang, D.-Y. Chen, H. Schatten, and Q.-Y. Sun Aurora-A Is a Critical Regulator of Microtubule Assembly and Nuclear Activity in Mouse Oocytes, Fertilized Eggs, and Early Embryos Biol Reprod, May 1, 2004; 70(5): 1392 - 1399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Fan and Q.-Y. Sun Involvement of Mitogen-Activated Protein Kinase Cascade During Oocyte Maturation and Fertilization in Mammals Biol Reprod, March 1, 2004; 70(3): 535 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Aumais, S. N. Williams, W. Luo, M. Nishino, K. A. Caldwell, G. A. Caldwell, S.-H. Lin, and L.-y. Yu-Lee Role for NudC, a dynein-associated nuclear movement protein, in mitosis and cytokinesis J. Cell Sci., May 15, 2003; 116(10): 1991 - 2003. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
