| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Department of Obstetrics and Gynecology, School of Medicine, Gunma University, Maebashi, Gunma 371-8511, Japan
b Department of Biochemistry, Fukui Medical University, Matsuoka, Fukui 910-1193 and
c CREST, JST (Japan Science and Technology Corporation), Japan
The present study was undertaken in order to identify the mechanism underlying the effect of transforming growth factor-ß (TGFß) on LH receptor (LH-R) expression in rat granulosa cells. Treatment with FSH produced a substantial increase in LH-R mRNA level, and concurrent treatment with increasing concentrations of TGFß brought about dose-dependent increases in FSH-induced LH receptor mRNA. TGFß, either alone or in combination with FSH, did not affect intracellular cAMP levels. We then investigated whether the effect of TGFß and FSH on LH-R mRNA levels results in increased transcription and/or altered mRNA stability. To determine whether the LH receptor 5'-flanking region plays a role in directing LH receptor mRNA expression, the proximal area of the LH receptor 5'-flanking regions were inserted into an expression vector, pGL-Basic, which contains luciferase as the receptor gene, and the resulting plasmids were transiently transfected into rat granulosa cells. FSH (30 ng/ml) significantly enhanced the activity of 1389 base pairs of the LH receptor 5'-flanking region, but treatment with TGFß did not significantly influence the activity induced by FSH. On the other hand, the decay curves for LH-R mRNA transcript in primary granulosa cells showed a significant increase in half-life after the addition of TGFß.
1 Supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (13470344), Tokyo, Japan, and by Fellowships of the Japan Society for the Promotion of Science for Japanese Junior Scientists (M.T.).
2 Correspondence. FAX: 81 27 220 8443; tminegis{at}showa.gunma-u.ac.jp
This article has been cited by other articles:
|
|
 |
|
  S. Ikeda, K. Nakamura, K. Kogure, Y. Omori, S. Yamashita, K. Kubota, T. Mizutani, K. Miyamoto, and T. Minegishi Effect of Estrogen on the Expression of Luteinizing Hormone-Human Chorionic Gonadotropin Receptor Messenger Ribonucleic Acid in Cultured Rat Granulosa Cells Endocrinology, April 1, 2008; 149(4): 1524 - 1533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. Chen, P.-W. Hsiao, M.-T. Lee, J I. Mason, F.-C. Ke, and J.-J. Hwang Interplay of PI3K and cAMP/PKA signaling, and rapamycin-hypersensitivity in TGF{beta}1 enhancement of FSH-stimulated steroidogenesis in rat ovarian granulosa cells J. Endocrinol., February 1, 2007; 192(2): 405 - 419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Omori, K. Nakamura, S. Yamashita, H. Matsuda, T. Mizutani, K. Miyamoto, and T. Minegishi Effect of Follicle-Stimulating Hormone and Estrogen on the Expression of Betaglycan Messenger Ribonucleic Acid Levels in Cultured Rat Granulosa Cells Endocrinology, August 1, 2005; 146(8): 3379 - 3386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-F. Kwok, W.-K. So, Y. Wang, and W. Ge Zebrafish Gonadotropins and Their Receptors: I. Cloning and Characterization of Zebrafish Follicle-Stimulating Hormone and Luteinizing Hormone Receptors-- Evidence for Their Distinct Functions in Follicle Development Biol Reprod, June 1, 2005; 72(6): 1370 - 1381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-C. Ke, S.-H. Fang, M.-T. Lee, S.-Y. Sheu, S.-Y. Lai, Y. J. Chen, F.-L. Huang, P. S Wang, D. M Stocco, and J.-J. Hwang Lindane, a gap junction blocker, suppresses FSH and transforming growth factor {beta}1-induced connexin43 gap junction formation and steroidogenesis in rat granulosa cells J. Endocrinol., March 1, 2005; 184(3): 555 - 566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-C. Ke, L.-C. Chuang, M.-T. Lee, Y. J. Chen, S.-W. Lin, P. S. Wang, D. M. Stocco, and J.-J. Hwang The Modulatory Role of Transforming Growth Factor {beta}1 and Androstenedione on Follicle-Stimulating Hormone-Induced Gelatinase Secretion and Steroidogenesis in Rat Granulosa Cells Biol Reprod, May 1, 2004; 70(5): 1292 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Inoue, K. Nakamura, K. Abe, T. Hirakawa, M. Tsuchiya, Y. Oomori, H. Matsuda, K. Miyamoto, and T. Minegishi Mechanisms of Action of Transforming Growth Factor {beta} on the Expression of Follicle-Stimulating Hormone Receptor Messenger Ribonucleic Acid Levels in Rat Granulosa Cells Biol Reprod, October 1, 2003; 69(4): 1238 - 1244. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
