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Regular Article |
a Monash Institute of Reproduction and Development
b The Centre for Bioprocess Technology, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Clayton, Victoria 3168, Australia
The phosphatidylethanolamine binding proteins (pebps) are an evolutionarily conserved family of proteins recently implicated in mitogen-activated protein (MAP) kinase pathway regulation, where they are called raf kinase inhibitory proteins. Here, we describe the cloning, cellular localization, and partial characterization of a new member, pebp-2, with potential roles in male fertility. Expression data show that pebp-2 is a testis-specific 21-kDa protein found within late meiotic and haploid germ cells in a stage-specific pattern that is temporally distinct from that of pebp-1. Sequence analyses suggest that pebp-2 forms a distinct subset of the pebp family within mammals. Database analyses revealed the existence of a third subset. Analysis suggests that the specificity/regulation of the distinct pebps subsets is likely to be determined by the amino terminal 40 amino acids or the 3' untranslated region, where the majority of sequence differences occur. Protein homology modeling suggests that pebp-2 protein is, however, topologically similar to other pebps and composed of Greek key fold motifs, a dominant ß-sheet formed from five anti-parallel ß strands forming a shallow groove associated with a putative phosphatidylethanolamine binding site. The pebp-2 gene is intronless and data suggest that it is a retrogene derived from pebp-1. Further, pebp-2 colocalizes with members of the MAP kinase pathway in late spermatocytes and spermatids and on the midpiece of epididymal sperm. These data raise the possibility that pebp-2 is a novel participant in the MAP kinase signaling pathway, with a role in spermatogenesis or posttesticular sperm maturation.
1 This work was initiated with funding from the Victorian Health Promotion Foundation and was supported by grants from the National Health and Medical Research Council (NH&MRC, 973218) of Australia. M.K.O.B. is the recipient of an R.D. Wright fellowship from the NH&MRC (143781).
2 Correspondence: Moira K. O'Bryan, Monash Institute of Reproduction and Development, Monash Medical Centre, 27-31 Wright St., Clayton, Victoria 3168, Australia. FAX: 61 3 9594 7111; moira.obryan{at}med.monash.edu.au
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