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Identification of Genes Encoding Mouse Oocyte Secretory and Transmembrane Proteins by a Signal Sequence Trap¹

^a Jackson Laboratory, Bar Harbor, Maine 04609

The oocyte plays a key role in follicular development. At all stages of follicular development, oocytes interact with surrounding granulosa cells and promote their differentiation into the types of cells that support further oocyte growth and developmental competence. These interactions suggest the existence of an oocyte-granulosa cell regulatory loop that includes both secreted proteins and cell surface receptors on both cell types. Factors involved in the regulatory loop will therefore contain a signal sequence, which can be used to identify them through a signal sequence trap (SST). A screen of an oocyte SST library identified three classes of oocyte-expressed sequences: known mouse genes, sequences homologous to known mammalian genes, and novel sequences of unknown function. Many of the recovered genes may have roles in the oocyte-granulosa cell regulatory loop. For several of the known mouse genes, new roles in follicular development are implied by identification of their expression, for the first time, in the oocyte. The future characterization of novel sequences may lead to the identification of novel proteins participating in the regulatory loop.

First decision: 5 April 2002.

¹ This research was supported by grant HD23839 (J.J.E.) from the NICHD. R.A.T. was supported by NIH NRSA grant F32 HD08730. Scientific Resources at the Jackson Laboratory are supported in part by a Cancer Center Core grant (CA34194) from the National Cancer Institute.

² Correspondence. FAX: 207 288 6073; jje{at}jax.org

³ These authors contributed equally

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