| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Department of Medical and Surgical Sciences, Clinica Medica 3, University of Padova, I-35128 Padova, Italy
b Department of Biochemistry, Technical University of Braunschweig, D-38124 Braunschweig, Germany
c Department of Biological Chemistry, University of Padova, I-35121 Padova, Italy
The selenoprotein phospholipid hydroperoxide glutathione peroxidase (PHGPx) accounts for almost the entire selenium content of mammalian testis. PHGPx is abundantly expressed in spermatids as active peroxidase but is transformed to an oxidatively inactivated protein in mature sperm, where it is a major constituent of the mitochondrial capsule in the midpiece. Male infertility in selenium-deficient animals, which is characterized by impaired sperm motility and morphological midpiece alterations, is considered to result from insufficient PHGPx content. We studied the relationship between sperm PHGPx, measured as rescued activity, and human fertility. Sperm specimens from 75 infertile men and 37 controls were analyzed for fertility-related parameters according to World Health Organization criteria. The PHGPx protein content was estimated after reductive solubilization of the spermatozoa by measuring the rescued PHGPx activity. Rescued PHGPx activity of infertile men ranged significantly below that of controls (93.2 ± 60.1 units/mg sperm protein vs. 187.5 ± 55.3 units/mg) and was particularly low in oligoasthenozoospermic specimens (61.93 ± 45.42 units/mg; P < 0.001 compared with controls and asthenozoospermic samples). Rescued PHGPx activity was correlated positively with viability, morphological integrity, and most profoundly forward motility (r = 0.35, 0.44, and 0.45, respectively). In isolated motile samples, motility decreased faster with decreasing PHGPx content. In humans, PHGPx appears to be indispensable for structural integrity of spermatozoa and to codetermine sperm motility and viability. Because the content of PHGPx, irrespective of the cause of alteration, is correlated with fertility-related parameters, PHGPx can be considered a predictive measure for fertilization capacity.
1 This work was supported by the Deutsche Forschungsgemeinschaft (grant Fl 61/12-1) and by the Italian Ministry of Education.
2 Correspondence: Matilde Maiorino, Department of Biological Chemistry, University of Padova, Viale G. Colombo, 3, I-35121 Padova, Italy. FAX: 39 049 8073310; mmaior{at}mail.bio.unipd.it
This article has been cited by other articles:
|
|
 |
|
  S. C. Schriever, K. M. Barnes, J. K. Evenson, A. M. Raines, and R. A. Sunde Selenium Requirements Are Higher for Glutathione Peroxidase-1 mRNA than Gpx1 Activity in Rat Testis Experimental Biology and Medicine, May 1, 2009; 234(5): 513 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Meplan, L. K Crosley, F. Nicol, G. W Horgan, J. C Mathers, J. R Arthur, and J. E Hesketh Functional effects of a common single-nucleotide polymorphism (GPX4c718t) in the glutathione peroxidase 4 gene: interaction with sex Am. J. Clinical Nutrition, April 1, 2008; 87(4): 1019 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Puglisi, A. Bevilacqua, G. Carlomagno, A. Lenzi, L. Gandini, M. Stefanini, F. Mangia, and C. Boitani Mice Overexpressing the Mitochondrial Phospholipid Hydroperoxide Glutathione Peroxidase in Male Germ Cells Show Abnormal Spermatogenesis and Reduced Fertility Endocrinology, September 1, 2007; 148(9): 4302 - 4309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Suzuki-Toyota, C. Ito, Y. Toyama, M. Maekawa, R. Yao, T. Noda, H. Iida, and K. Toshimori Factors Maintaining Normal Sperm Tail Structure During Epididymal Maturation Studied in Gopc / Mice Biol Reprod, July 1, 2007; 77(1): 71 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu, L. E. Dettin, J. Folmer, B. R. Zirkin, and V. Papadopoulos Abnormal Morphology of Spermatozoa in Cytochrome P450 17{alpha}-hydroxylase/17, 20-lyase (CYP17) Deficient Mice J Androl, May 1, 2007; 28(3): 453 - 460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Olson, V. P. Winfrey, S. K. NagDas, K. E. Hill, and R. F. Burk Apolipoprotein E Receptor-2 (ApoER2) Mediates Selenium Uptake from Selenoprotein P by the Mouse Testis J. Biol. Chem., April 20, 2007; 282(16): 12290 - 12297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Moghadaszadeh and A. H. Beggs Selenoproteins and Their Impact on Human Health Through Diverse Physiological Pathways. Physiology, October 1, 2006; 21(5): 307 - 315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kohrle, F. Jakob, B. Contempre, and J. E. Dumont Selenium, the Thyroid, and the Endocrine System Endocr. Rev., December 1, 2005; 26(7): 944 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Scimeca, D. J. Lisk, T. Prolla, and X. G. Lei Effects of gpx4 Haploid Insufficiency on GPx4 Activity, Selenium Concentration, and Paraquat-Induced Protein Oxidation in Murine Tissues Experimental Biology and Medicine, November 1, 2005; 230(10): 709 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Su, S. V. Novoselov, Q.-A. Sun, M. E. Moustafa, Y. Zhou, R. Oko, D. L. Hatfield, and V. N. Gladyshev Mammalian Selenoprotein Thioredoxin-glutathione Reductase: ROLES IN DISULFIDE BOND FORMATION AND SPERM MATURATION J. Biol. Chem., July 15, 2005; 280(28): 26491 - 26498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. Olson, V. P. Winfrey, S. K. NagDas, K. E. Hill, and R. F. Burk Selenoprotein P Is Required for Mouse Sperm Development Biol Reprod, July 1, 2005; 73(1): 201 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lapointe, S. Kimmins, L. A. MacLaren, and J.-F. Bilodeau Estrogen Selectively Up-Regulates the Phospholipid Hydroperoxide Glutathione Peroxidase in the Oviducts Endocrinology, June 1, 2005; 146(6): 2583 - 2592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Lyi, L. I. Heller, M. Rutzke, R. M. Welch, L. V. Kochian, and L. Li Molecular and Biochemical Characterization of the Selenocysteine Se-Methyltransferase Gene and Se-Methylselenocysteine Synthesis in Broccoli Plant Physiology, May 1, 2005; 138(1): 409 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J Beckett and J. R Arthur Selenium and endocrine systems J. Endocrinol., March 1, 2005; 184(3): 455 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. NagDas, V. P. Winfrey, and G. E. Olson Tyrosine Phosphorylation Generates Multiple Isoforms of the Mitochondrial Capsule Protein, Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx), During Hamster Sperm Capacitation Biol Reprod, January 1, 2005; 72(1): 164 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wenk, J. Schuller, C. Hinrichs, T. Syrovets, N. Azoitei, M. Podda, M. Wlaschek, P. Brenneisen, L.-A. Schneider, A. Sabiwalsky, et al. Overexpression of Phospholipid-hydroperoxide Glutathione Peroxidase in Human Dermal Fibroblasts Abrogates UVA Irradiation-induced Expression of Interstitial Collagenase/Matrix Metalloproteinase-1 by Suppression of Phosphatidylcholine Hydroperoxide-mediated NF{kappa}B Activation and Interleukin-6 Release J. Biol. Chem., October 29, 2004; 279(44): 45634 - 45642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Meseguer, N. Garrido, C. Simon, A. Pellicer, and J. Remohi Concentration of Glutathione and Expression of Glutathione Peroxidases 1 and 4 in Fresh Sperm Provide a Forecast of the Outcome of Cryopreservation of Human Spermatozoa J Androl, September 1, 2004; 25(5): 773 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E Olson, V. P Winfrey, K. E Hill, and R. F Burk Sequential development of flagellar defects in spermatids and epididymal spermatozoa of selenium-deficient rats Reproduction, March 1, 2004; 127(3): 335 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maiorino, M. Scapin, F. Ursini, M. Biasolo, V. Bosello, and L. Flohe Distinct Promoters Determine Alternative Transcription of gpx-4 into Phospholipid-Hydroperoxide Glutathione Peroxidase Variants J. Biol. Chem., September 5, 2003; 278(36): 34286 - 34290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Haraguchi, T. Mabuchi, S. Hirata, T. Shoda, A. T. Yamada, K. Hoshi, and S. Yokota Spatiotemporal Changes of Levels of a Moonlighting Protein, Phospholipid Hydroperoxide Glutathione Peroxidase, in Subcellular Compartments During Spermatogenesis in the Rat Testis Biol Reprod, September 1, 2003; 69(3): 885 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Andersen, C.-H. Yeung, H. Vorum, M. Wellner, T. K. Andreassen, B. Erdmann, E.-C. Mueller, J. Herz, A. Otto, T. G. Cooper, et al. Essential Role of the Apolipoprotein E Receptor-2 in Sperm Development J. Biol. Chem., June 20, 2003; 278(26): 23989 - 23995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maiorino, V. Bosello, F. Ursini, C. Foresta, A. Garolla, M. Scapin, H. Sztajer, and L. Flohe Genetic Variations of gpx-4 and Male Infertility in Humans Biol Reprod, April 1, 2003; 68(4): 1134 - 1141. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
