| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Regular Article |
a Center for Research on Reproduction and Women's Health and Department of Obstetrics & Gynecology, University of Pennsylvania, Philadelphia, Pennsylvania 19104
In the mammalian oocyte, the cAMP-dependent protein kinase (PKA) has critical functions in the maintenance of meiotic arrest and oocyte maturation. Because PKA is spatially regulated, its localization was examined in developing oocytes. Both regulatory subunits (RI and RII) and the catalytic subunit (C) of PKA were found in oocytes and metaphase II-arrested eggs. In the oocyte, RI and C were predominantly localized in the cortical region, while RII showed a punctate distribution within the cytoplasm. After maturation to metaphase II, RI remained in the cortex and was also localized to the meiotic spindle, while RII was found adjacent to the spindle. C was diffuse within the cytoplasm of the egg but was enriched in the cytoplasm surrounding the metaphase spindle, much like RII. The polarized localization and redistribution of RI, RII, and C suggested that PKA might be tethered by A-kinase anchor proteins (AKAPs), proteins that tether PKA close to its physiological substrates. An AKAP, AKAP140, was identified that was developmentally regulated and phosphorylated in oocytes and eggs. AKAP140 was shown to be a dual-specific AKAP, having the ability to bind both RI and RII. By compartmentalizing PKA, AKAP140 and/or other AKAPs could spatially regulate PKA activity during oocyte development.
1 This work was supported by a Howard Hughes Medical Institute Research Training Fellowship to R.L.B., a Burroughs Wellcome Fund Career Award in the Biomedical Sciences to C.J.W., and NIH PO1 HD06274 to S.B.M. Part of this work was done in the Intermediate Voltage Electron Microscopy and Biomedical Image Analysis Facility at the University of Pennsylvania, supported by NIH grant RR-2483.
2 Correspondence: Carmen J. Williams, Center for Research on Reproduction & Women's Health, 1313 BRB II/III, 421 Curie Blvd., Philadelphia, PA 19104-6142. FAX: 215-573-7627; cjwill{at}mail.med.upenn.edu
3 Current address: Department of Medicine, University of Chicago Hospitals, 5841 S. Maryland Ave., Chicago, IL 60637-1419
4 These authors contributed equally to the work described in this manuscript
This article has been cited by other articles:
|
|
 |
|
  G. Ning, H. Ouyang, S. Wang, X. Chen, B. Xu, J. Yang, H. Zhang, M. Zhang, and G. Xia 3',5'-Cyclic Adenosine Monophosphate Response Element Binding Protein Up-Regulated Cytochrome P450 Lanosterol 14{alpha}-Demethylase Expression Involved in Follicle-Stimulating Hormone-Induced Mouse Oocyte Maturation Mol. Endocrinol., July 1, 2008; 22(7): 1682 - 1694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kovo, M. Kandli-Cohen, M. Ben-Haim, D. Galiani, D. W Carr, and N. Dekel An active protein kinase A (PKA) is involved in meiotic arrest of rat growing oocytes Reproduction, July 1, 2006; 132(1): 33 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jamnongjit, A. Gill, and S. R. Hammes Epidermal growth factor receptor signaling is required for normal ovarian steroidogenesis and oocyte maturation PNAS, November 8, 2005; 102(45): 16257 - 16262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gill, M. Jamnongjit, and S. R. Hammes Androgens Promote Maturation and Signaling in Mouse Oocytes Independent of Transcription: A Release of Inhibition Model for Mammalian Oocyte Meiosis Mol. Endocrinol., January 1, 2004; 18(1): 97 - 104. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
