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Multiple Trp Isoforms Implicated in Capacitative Calcium Entry Are Expressed in Human Pregnant Myometrium and Myometrial Cells¹

^a Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, Houston, Texas 77030

Capacitative Ca²⁺ entry plays a role in thapsigargin- and oxytocin-mediated increases in intracellular free Ca²⁺ in human myometrium. Members of the Trp protein family have been implicated in capacitative Ca²⁺ entry in a number of tissues. Pregnant human myometrium and the human myometrial cell line PHM1-41 expressed mRNA for hTrp1, hTrp3, hTrp4, hTrp6, and hTrp7. A number of known splice variants of hTrp1 and hTrp4 were expressed in these cells. In addition, novel splice variants for hTrp1 and hTrp3 were discovered. hTrp11 and hTrp12 contain insertions between previously described exons 9 and 10 that would alter reading frame and produce Trp proteins truncated in the membrane spanning region if expressed. The hTrp3 variant introduces sequence between exons 8 and 9 that would insert 16 amino acids in the C-terminal region of the protein upstream of the calmodulin and inositol 1,4,5-triphosphate receptor interaction domain. hTrp1, hTrp3, and hTrp4 proteins were detected in both pregnant human myometrial and PHM1-41 membranes; a weak band consistent with hTrp6 expression was detected in pregnant human myometrium. These data are consistent with the presence of proteins that could form putative capacitative Ca²⁺ channels in human myometrium. Control of the activity of these channels may be important for the control of uterine contractile activity.

First decision: 25 February 2002.

¹ This work was supported in part by NIH-HD09618, NIH-HD38970, and Lalor Foundation Fellowships (M.Y. and A.G.).

² Correspondence: Barbara M. Sanborn, Department of Biochemistry and Molecular Biology, University of Texas Medical School, P.O. Box 20708, Houston, TX 77225. FAX: 713 500 0652; barbara.m.sanborn{at}uth.tmc.edu

³ Current address: Lexicon Genetics, 4000 Research Forest Dr., The Woodlands, TX 77381

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