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Expression of Calcium Channels along the Differentiation of Cultured Trophoblast Cells from Human Term Placenta¹

^a Laboratoire de Physiologie materno-foetale, Département des Sciences Biologiques, Université du Québec à Montréal, Montréal, Québec, Canada H3C 3P8

Placental transfer of maternal calcium (Ca²⁺) is carried out in vivo by the syncytiotrophoblast layer. Although this process is crucial for fetal development, it remains poorly understood. Cytotrophoblasts isolated from human term placenta undergo spontaneous syncytiotrophoblast-like morphological and biochemical differentiation in vitro and are thought to reflect in vivo syncytiotrophoblast. In the present study, we characterized the Ca²⁺ uptake potential and the expression of several Ca²⁺ channels by human trophoblasts during differentiation in vitro for up to 6 days. Secretion of hCG (specific differentiation marker) and uptake of Ca²⁺ by trophoblasts increased gradually as a function of days in culture. Both hCG secretion and Ca²⁺ uptake were maximal on Day 4 and declined on Days 5–6. Expression of the Ca²⁺ transporter proteins CaT1 and CaT2 was revealed by reverse transcription-polymerase chain reaction in cytotrophoblasts freshly isolated from human term placenta. In addition, messengers for two L-type Ca²⁺ channel isoforms (_1C and _1D) were also detected. Levels of CaT1, CaT2, and L-type Ca²⁺ channel mRNA increased gradually during culture, reaching a maximum between Days 2 and 3. In contrast to CaT1 and CaT2 expression that declined thereafter to levels observed on Day 1, L-type channel expression decreased by 50% but remained above the expression level of Day 1. Our results indicate that the pattern of CaT1 and CaT2 expression correlates with the Ca²⁺ uptake potential along the differentiation of cultured human trophoblasts isolated from term placenta. This correlation provides circumstantial evidence for a role of this family of channels in basal Ca²⁺ uptake by the syncytiotrophoblast.

¹ Supported by a grant from the March of Dimes, Saving Babies Together.

² Correspondence: Julie Lafond, Département des Sciences Biologiques, Laboratoire de Physiologie materno-ftale, Université du Québec à Montréal, C.P. 8888, Succursale "Centre-Ville", Montréal, QC, Canada H3C 3P8. FAX: 514 987 4647; lafond.julie{at}uqam.ca

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