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BOR - Papers in Press, published online ahead of print October 4, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.005207
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biolreprod.102.005207v1
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Biology of Reproduction 67, 1832-1839 (2002)
DOI: 10.1095/biolreprod.102.005207 © 2002 Society for the Study of Reproduction, Inc.

Male Reproductive Tract

Expression and Location of the Bradykinin B2 Receptor in Rat Testis1

Thomas K. Monseesa, Sonja Blöchera, Frank Heidornb, Anett Winklerc, Wolf-Eberhard Siemsc, Werner Müller-Esterld, Jaleh Hayatpoura, Werner Miskaa, and Wolf-Bernhard Schilla

a Center for Dermatology and Andrology, Justus Liebig University, D-35385 Giessen, Germany b Institute for Legal Medicine, Justus Liebig University, D-35385 Giessen, Germany c Institute for Molecular Pharmacology, D-10315 Berlin, Germany d Institute for Biochemistry II, Johann Wolfgang Goethe University, D-60590 Frankfurt, Germany

To investigate the possible role of the local tissue kallikrein-kinin system in spermatogenesis, we analyzed gene expression and cellular distribution of the bradykinin subtype-2 receptor (B2 receptor) in the rat testis. Reverse transcription-polymerase chain reaction revealed B2 receptor expression in testis and primary cultures of Sertoli cells and peritubular cells isolated from immature and mature rats. In situ hybridization of the B2-receptor mRNA showed intense labeling of cells on the base of the seminiferous tubule, whereas the autoradiographic signals gradually decreased toward the lumen. Immune histochemistry using testicular sections of pubertal and adult rats showed specific staining for the B2-receptor protein in cells of the adluminal compartment of the seminiferous tubules, especially on pachytene spermatocytes and spermatids. This immunostaining varied with the stages of the seminiferous cycle. The receptor protein was also observed on peritubular cells of pubertal rats. In conclusion, we demonstrated a stage-specific expression of the bradykinin B2 receptor in different cells of the seminiferous tubules of the rat testis. The results point to a possible function of the tissue kallikrein-kinin system in the local regulation of spermatogenesis.

1 Supported by the Deutsche Forschungsgemeinschaft (DFG Mo 693/3-1).

2 Correspondence: Thomas K. Monsees, Center for Dermatology and Andrology, Justus Liebig University of Giessen, Gaffkystr. 14, D-35385 Giessen, Germany. FAX: 49 641 99 43379; thomas.monsees{at}derma.med.uni-giessen.de

3 These authors contributed equally to this work




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