Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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BOR - Papers in Press, published online ahead of print October 4, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.004572
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Biology of Reproduction 67, 1864-1871 (2002)
DOI: 10.1095/biolreprod.102.004572 © 2002 Society for the Study of Reproduction, Inc.

Neuroendocrinology

Expression of Pejerrey Gonadotropin-Releasing Hormone in Three Orders of Fish1

G.M. Somoza2,a, D.W. Lescheidb, L.A. Mirandaa, F.L. Lo Nostroc, L. Magliulo-Ceprianod, A.D. Montanere, M.P. Schreibmand, J.E. Rivierf, and N.M. Sherwoodb

a Instituto Tecnológico de Chascomús (IIB-INTECH, CONICET-Universidad de San Martín), Camino de Circunvalación Laguna, Km 6. CC 164 (B7130IWA) Chascomús, Provincia de Buenos Aires, Argentina b Department of Biology, University of Victoria, Victoria, British Columbia, Canada V8W 2Y2 c Laboratorio de Embriología Animal, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (C1428EHA), Buenos Aires, Argentina d Aquatic Research and Environmental Assessment Center (AREAC), Brooklyn College of the City University> of New York, New York, New York 11210 e Instituto de Investigaciones Biomédicas, Fundación Pablo Cassará, Saladillo 2452 (C1440FFX), Buenos Aires, Argentina f The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, La Jolla, California 92037

Molecular variants of GnRH were characterized by reverse-phase, high-performance liquid chromatography from brain extracts of fish in three different orders: Synbranchiformes (swamp eel [Synbranchus marmoratus]), Cyprinidontiformes (platyfish [Xiphophorus maculatus] and green swordtail [X. helleri]), and Atheriniformes (Patagonia pejerrey [Odontesthes hatchery]). Also, pituitary gland extracts from the pejerrey O. bonariensis (Atheriniformes) were characterized. Eluted fractions were tested in radioimmunoassays with antisera specific to GnRH, including both antisera that detected only one form of GnRH and those that detected several forms. The results show that brain extracts obtained from all species contained the same three molecular forms of GnRH, which were immunologically and chromatographically undistinguishable from chicken GnRH-II, pejerrey GnRH (pjGnRH), and salmon GnRH. This study supports the hypothesis that expression of these three forms is common in different fish orders and that pjGnRH is the main regulator of pituitary function in these fish.

1 Funded by the Canadian Institutes of Health Research (MOP-8916), ANPCYT (Argentina, PICT 01-04424), and Fundación Antorchas (Argentina).

2 Correspondence. FAX: 54 2241 424048; somoza{at}intech.gov.ar






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