| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Ovary |
a Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853
In mice deficient in progesterone receptor (PR), follicles of ovulatory size develop but fail to ovulate, providing evidence for an essential role for progesterone and PR in ovulation in mice. However, little is known about the expression and regulation of PR mRNA in preovulatory follicles of ruminant species. One objective of this study was to determine whether and when PR mRNA is expressed in bovine follicular cells during the periovulatory period. Luteolysis and the LH/FSH surge were induced with prostaglandin F2
and a GnRH analogue, respectively, and the preovulatory follicle was obtained at 0, 3.5, 6, 12, 18, or 24 h after GnRH treatment. RNase protection assays revealed a transient increase in levels of PR mRNA, which peaked at 6 h after GnRH and declined to the time 0 value by 12 h and a second increase at 24 h. The second objective was to investigate the mechanisms that regulate PR mRNA expression through in vitro studies on follicular cells of preovulatory follicles obtained before the LH/FSH surge. Theca and granulosa cells were isolated and cultured with or without a luteinizing dose of LH or FSH, progesterone, LH + progesterone, or LH + antiprogestin (RU486). Levels of PR mRNA increased in a time-dependent manner in granulosa cells cultured with LH or FSH and in theca cells cultured with LH, peaking at 10 h of culture. In contrast, progesterone (200 ng/ml) did not upregulate mRNA for its own receptor, and neither progesterone nor RU486 affected LH-stimulated PR mRNA accumulation. Furthermore, RU486 completely blocked LH-stimulated expression of oxytocin mRNA, indicating that PR induced by LH in vitro is functional. These results show that the gonadotropin surge induces a rapid and transient increase in expression of PR mRNA in both theca and granulosa cells of bovine periovulatory follicles followed by a second rise close to the time of ovulation and that the first increase in PR mRNA can be mimicked in vitro by gonadotropins but not by progesterone. These results suggest multiple and time-dependent roles for progesterone and PR in the regulation of periovulatory events in cattle.
2 Correspondence: J.E. Fortune, T6-012B VRT, Cornell University, Ithaca, NY 14853. FAX: 607 253 3476; jf11{at}cornell.edu
3 Current address: Department of Obstetrics and Gynecology, University of Kentucky, Lexington, KY 40536-0293
4 Current address: Department of Animal Science, Iowa State University, Ames, IA 50011-3150
This article has been cited by other articles:
|
|
 |
|
  E. Klipper, E. Tatz, T. Kisliouk, I. Vlodavsky, U. Moallem, D. Schams, Y. Lavon, D. Wolfenson, and R. Meidan Induction of Heparanase in Bovine Granulosa Cells by Luteinizing Hormone: Possible Role during the Ovulatory Process Endocrinology, January 1, 2009; 150(1): 413 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Li, F. Jimenez-Krassel, A. Bettegowda, J. J Ireland, and G. W Smith Evidence that the preovulatory rise in intrafollicular progesterone may not be required for ovulation in cattle J. Endocrinol., March 1, 2007; 192(3): 473 - 483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Teilmann, C. A. Clement, J. Thorup, A. G. Byskov, and S. T. Christensen Expression and localization of the progesterone receptor in mouse and human reproductive organs J. Endocrinol., December 1, 2006; 191(3): 525 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Bridges, C. M. Komar, and J. E. Fortune Gonadotropin-Induced Expression of Messenger Ribonucleic Acid for Cyclooxygenase-2 and Production of Prostaglandins E and F2{alpha} in Bovine Preovulatory Follicles Are Regulated by the Progesterone Receptor Endocrinology, October 1, 2006; 147(10): 4713 - 4722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jo and T. E. Curry Jr. Luteinizing Hormone-Induced RUNX1 Regulates the Expression of Genes in Granulosa Cells of Rat Periovulatory Follicles Mol. Endocrinol., September 1, 2006; 20(9): 2156 - 2172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Banerjee and C. M Komar Effects of luteinizing hormone on peroxisome proliferator-activated receptor {gamma} in the rat ovary before and after the gonadotropin surge Reproduction, January 1, 2006; 131(1): 93 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B Lohrke, T Viergutz, and B Kruger Polar phospholipids from bovine endogenously oxidized low density lipoprotein interfere with follicular thecal function J. Mol. Endocrinol., December 1, 2005; 35(3): 531 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A Stoops, R. D Pairan, and T. L Roth Follicular, endocrine and behavioural dynamics of the Indian rhinoceros (Rhinoceros unicornis) oestrous cycle Reproduction, December 1, 2004; 128(6): 843 - 856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jo and T. E. Curry Jr Regulation of Matrix Metalloproteinase-19 Messenger RNA Expression in the Rat Ovary Biol Reprod, December 1, 2004; 71(6): 1796 - 1806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Li, L. J Bakke, J R. Pursley, and G. W Smith Localization and temporal regulation of tissue inhibitors of metalloproteinases 3 and 4 in bovine preovulatory follicles Reproduction, November 1, 2004; 128(5): 555 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Quirk, R. G. Cowan, and R. M. Harman Progesterone Receptor and the Cell Cycle Modulate Apoptosis in Granulosa Cells Endocrinology, November 1, 2004; 145(11): 5033 - 5043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jo, M. C. Gieske, C. E. Payne, S. E. Wheeler-Price, J. B. Gieske, I. V. Ignatius, T. E. Curry Jr., and C. Ko Development and Application of a Rat Ovarian Gene Expression Database Endocrinology, November 1, 2004; 145(11): 5384 - 5396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Brown, D. Boerboom, N. Bouchard, M. Dore, J. G. Lussier, and J. Sirois Human Chorionic Gonadotropin-Dependent Regulation of 17{beta}-Hydroxysteroid Dehydrogenase Type 4 in Preovulatory Follicles and Its Potential Role in Follicular Luteinization Endocrinology, April 1, 2004; 145(4): 1906 - 1915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jo, L. E. Thomas, S. E. Wheeler, and T. E. Curry Jr Membrane Type 1-Matrix Metalloproteinase (MMP)-Associated MMP-2 Activation Increases in the Rat Ovary in Response to an Ovulatory Dose of Human Chorionic Gonadotropin Biol Reprod, April 1, 2004; 70(4): 1024 - 1032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Quirk, R. G. Cowan, R. M. Harman, C.-L. Hu, and D. A. Porter Ovarian follicular growth and atresia: The relationship between cell proliferation and survival J Anim Sci, January 1, 2004; 82(13_suppl): E40 - 52. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
