Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print October 4, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.003673
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
67/6/1989    most recent
biolreprod.102.003673v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vitale, A. M.
Right arrow Articles by Tesone, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vitale, A. M.
Right arrow Articles by Tesone, M.
Agricola
Right arrow Articles by Vitale, A. M.
Right arrow Articles by Tesone, M.
Biology of Reproduction 67, 1989-1995 (2002)
DOI: 10.1095/biolreprod.102.003673 © 2002 Society for the Study of Reproduction, Inc.

Ovary

Inhibin A Increases Apoptosis in Early Ovarian Antral Follicles of Diethylstilbestrol-Treated Rats1

Alejandra M. Vitalea, Olga M. Gonzalezb, Fernanda Parborella, Griselda Irustaa, Stella Campoc, and Marta Tesone2,a

a Instituto de Biología y Medicina Experimental CONICET, b Facultad de Ciencias Exactas y Naturales, Facultad de Agronomía, c Departamento de Producción Animal, Universidad de Buenos Aires, and Centro de Investigaciones Endocrinológicas, Buenos Aires 1428, Argentina

The purpose of this study was to evaluate the role of inhibin A in follicular development and apoptosis-related mechanisms in preantral and early antral follicles from prepubertal diethylstilbestrol (DES)-treated rats. Granulosa cells isolated from the ovaries of 23- to 25-day-old rats were cultured in serum-free medium containing FSH (20 ng/ml), transforming growth factor ß (5 ng/ml), and estradiol (50 ng/ml) in the presence or absence of different concentrations of recombinant human inhibin A. 3H-Thymidine incorporation was decreased in the presence of Inh, but no significant changes were observed in progesterone and estradiol levels in culture medium. An increase in low molecular weight DNA fragmentation indicative of apoptosis and an increase in the levels of Bax protein with no changes in Bcl-2 protein levels were evident in early antral follicles incubated for 24 h with Inh. For each animal, Inh (0.5 µg/ovary) was injected intrabursally in one ovary, and the contralateral ovary served as a control. Ovarian histology revealed an inhibitory effect of Inh treatment on the follicular development induced by DES. At 24 h after Inh injection, the number of preantral follicles was increased compared with controls, whereas the number of early antral follicles was decreased. In addition, in vivo Inh treatment caused an increase in the percentage of apoptotic cells in preantral and early antral follicles. These results suggest that inhibin produced by the dominant follicle may act as a paracrine factor inhibiting the growth of neighboring follicles, thus participating in the mechanism of follicular selection.

1 This study was supported by the ANPCYT (PICT 6384 BID 1201 OC-AR), UBA (01/TW05, University of Buenos Aires, Argentina), and the Roemmers Foundation.

2 Correspondence: Marta Tesone, Instituto de Biología y Medicina Experimental, Obligado 2490, Buenos Aires 1428, Argentina. FAX: 54 011 4786 2564; mtesone{at}dna.uba.ar






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Society for the Study of Reproduction.