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This Article Full Text Full Text (PDF) All Versions of this Article: 68/1/136    most recent biolreprod.102.008706v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kaneko, T. Articles by Yanagimachi, R. Search for Related Content PubMed PubMed Citation Articles by Kaneko, T. Articles by Yanagimachi, R. Agricola Articles by Kaneko, T. Articles by Yanagimachi, R.

Effect of pH Value of Freeze-Drying Solution on the Chromosome Integrity and Developmental Ability of Mouse Spermatozoa¹

^a The Institute for Biogenesis Research, Department of Anatomy and Reproductive Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822 ^b Division of Reproductive Engineering, Center for Animal Resources and Development, Kumamoto University, Kumamoto 860-0811, Japan

The nuclei of freeze-dried mouse spermatozoa are able to retain their chromosome integrity and developmental potential. To optimize the conditions of freeze-drying, we examined whether pH values of the freeze-drying solution affect the chromosome integrity and developmental potential of sperm nuclei. The sperm freeze-drying solution we used contained a high concentration (50 mM) of calcium-chelating EGTA. Sperm chromosomes were examined at the metaphase of the first mitosis after injection of freeze-dried spermatozoa into matured oocytes. The developmental potential of sperm nuclei was assessed by examining the development of fetuses in midgestation. The results showed that both sperm chromosomes and sperm developmental potential are maintained better when the freeze-drying solution was slightly alkaline (pH 8.0) rather than near neutral or acidic (pH 7.4–6.0). The data indicated that the chromosome integrity and developmental ability of mouse spermatozoa are affected by the pH value of freeze-drying solution.

¹ This study was conducted as part of the National Cooperative Program on Mouse Sperm Cryopreservation sponsored by the National Institute of Child Health and Human Development and the National Center for Research Resources (U01HD38205).

² Correspondence: Takehito Kaneko, The Institute for Biogenesis Research, Department of Anatomy and Reproductive Biology, John A. Burns School of Medicine, University of Hawaii, 1960 East-West Road, Honolulu, HI 96822. FAX: 808 956 7316; takehito{at}hawaii.edu

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