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BOR - Papers in Press, published online ahead of print October 14, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.008516
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BIOLOGY OF REPRODUCTION 68, 167–173 (2003)
DOI: 10.1095/biolreprod.102.008516
© 2003 by the Society for the Study of Reproduction, Inc.

Reproductive Technology

Allogeneic Offspring Produced by Male Germ Line Stem Cell Transplantation into Infertile Mouse Testis1

Mito Kanatsu-Shinoharaa, Narumi Ogonukic, Kimiko Inouec, Atsuo Ogurac, Shinya Toyokunib, Tasuku Honjoa, and Takashi Shinohara2,a

a Department of Medical Chemistry, b Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan 606-8501 c The Institute of Physical and Chemical Research (RIKEN), Bioresource Center, Ibaraki, Japan 305-0074

The testis is one of several immune-privileged organs and is known for its unique ability to support allogeneic or xenogeneic tissue transplants. We investigated the possibility of deriving offspring from mice that underwent transplantation with allogeneic male germ line stem cells in the testis. Although mature adult mice rejected allogeneic germ cells and were infertile, offspring were obtained by intracytoplasmic germ cell injection using partially differentiated donor cells. In contrast, complete spermatogenesis occurred when allogeneic germ cells were transplanted into immature pup testes. Tolerance induction by monoclonal antibody administration allowed the pup transplant recipients to produce allogeneic offspring by natural mating, whereas no spermatozoa were found in the epididymis of untreated recipients. Thus, these results indicate that a histoincompatible recipient can serve as a "surrogate father" to propagate the genetic information of heterologous male donors.

1 Supported by the Kanae Foundation for Life & Socio-Medical Science and the Ministry of Education, Science, Sports, and Culture of Japan.

2 Correspondence: Takashi Shinohara, Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Yoshida-Konoe, Sakyo-ku, Kyoto, Japan 606-8501. FAX: 81 75 753 4388; takashi{at}mfour.med.kyoto-u.ac.jp






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Copyright © 2003 by the Society for the Study of Reproduction.