|
|
||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Gamete Biology |
a Centre de Recherche en Biologie de la Reproduction, Département des Sciences Animales, Université Laval, Sainte-Foy, Québec, Canada G1K 7P4
Mammalian sperm undergo capacitation in the female reproductive tract or under defined conditions in vitro. Although capacitation is now considered to be mediated by intracellular signaling events, including protein phosphorylation, the regulation of the transduction mechanisms is poorly understood. The objective of the present study was to evaluate the importance of medium components on capacitation of porcine sperm, the appearance of an Mr 32 000 sperm protein (p32), and activity of a tyrosine kinase (TK-32). As determined by the ability of the sperm to undergo the A23187-induced acrosome reaction, pig sperm require bicarbonate and calcium but not BSA for capacitation in vitro. The appearance of p32 was assessed by immunoblotting SDS-extracted and separated sperm proteins using an anti-phosphotyrosine antibody. The appearance of p32 requires calcium, although p32 appears even in the absence of bicarbonate in the incubation medium, demonstrating that the appearance of this tyrosine phosphoprotein is not a final end point of pig sperm capacitation. An in-gel tyrosine kinase renaturation assay showed that TK-32 activity depends on calcium and bicarbonate in the incubation medium. Immunoprecipitation experiments using an anti-phosphotyrosine antibody and inhibitor demonstrated that p32 and TK-32 are different proteins. These data indicate that the signal transduction mechanisms of capacitation in pig sperm are different from those in other mammals, suggesting that certain species specificity may exist with respect to this phenomenon.
2 Correspondence: Janice L. Bailey, Centre de Recherche en Biologie de la Reproduction, Département des Sciences Animales, Pavillon Paul-Comtois, Université Laval, Sainte-Foy, QC, Canada G1K 7P4. FAX: 481 656 3766; janice.bailey{at}crbr.ulaval.ca
This article has been cited by other articles:
![]() |
C. Campagna, C. Guillemette, P. Ayotte, and J. L. Bailey Effects of an Environmentally Relevant Organochlorine Mixture and a Metabolized Extract of This Mixture on Porcine Sperm Parameters In Vitro J Androl, May 1, 2009; 30(3): 317 - 324. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Aquila, V. Rago, C. Guido, I. Casaburi, S. Zupo, and A. Carpino Leptin and leptin receptor in pig spermatozoa: evidence of their involvement in sperm capacitation and survival Reproduction, July 1, 2008; 136(1): 23 - 32. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. L. Vadnais and K. P. Roberts Effects of Seminal Plasma on Cooling-Induced Capacitative Changes in Boar Sperm J Androl, May 1, 2007; 28(3): 416 - 422. [Abstract] [Full Text] [PDF] |
||||
![]() |
M.-F. Lusignan, A. Bergeron, M.-H. Crete, C. Lazure, and P. Manjunath Induction of Epididymal Boar Sperm Capacitation by pB1 and BSP-A1/-A2 Proteins, Members of the BSP Protein Family Biol Reprod, March 1, 2007; 76(3): 424 - 432. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. Grasa, J. A. Cebrian-Perez, and T. Muino-Blanco Signal transduction mechanisms involved in in vitro ram sperm capacitation. Reproduction, November 1, 2006; 132(5): 721 - 732. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Dube, P. Leclerc, T. Baba, C. Reyes-Moreno, and J. L. Bailey The Proacrosin Binding Protein, sp32, Is Tyrosine Phosphorylated During Capacitation of Pig Sperm J Androl, July 1, 2005; 26(4): 519 - 528. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Shadan, P. S. James, E. A. Howes, and R. Jones Cholesterol Efflux Alters Lipid Raft Stability and Distribution During Capacitation of Boar Spermatozoa Biol Reprod, July 1, 2004; 71(1): 253 - 265. [Abstract] [Full Text] [PDF] |
||||
![]() |
V. G. Da Ros, M. J. Munuce, D. J. Cohen, C. I. Marin-Briggiler, D. Busso, P. E. Visconti, and P. S. Cuasnicu Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability Biol Reprod, May 1, 2004; 70(5): 1325 - 1332. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Harayama Viability and Protein Phosphorylation Patterns of Boar Spermatozoa Agglutinated by Treatment With a Cell-Permeable Cyclic Adenosine 3',5'-Monophosphate Analog J Androl, November 1, 2003; 24(6): 831 - 842. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. C. Kirkman-Brown Editorial Commentary J Androl, September 1, 2003; 24(5): 734 - 735. [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |