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BOR - Papers in Press, published online ahead of print October 14, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.005645
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BIOLOGY OF REPRODUCTION 68, 302–308 (2003)
DOI: 10.1095/biolreprod.102.005645
© 2003 by the Society for the Study of Reproduction, Inc.

Female Reproductive Tract

Expression, Immunolocalization, and Functional Activity of Na+/H+ Exchanger Isoforms in Mouse Endometrial Epithelium1

X.F. Wanga, M.K. Yua, S.Y. Lama, K.M. Leunga, J.L. Jianga, P.S. Leunga, W.H. Koa, P.Y. Leunga, S.B.C. Chewa, C.Q. Liub, C.M. Tsec, and H.C. Chan2,a

a Department of Physiology, The Chinese University of Hong Kong, Shatin, Hong Kong b Shanghai Institute of Planned Parenthood Research, Shanghai, China c Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205

The luminal fluid microenvironment of the uterus is important for sperm capacitation and embryo development. In an attempt to understand the possible role of Na+/H+ exchangers (NHEs) in uterine function, the mRNAs of different NHE isoforms as well as their subcellular localization (apical versus basolateral) and functional activity were investigated in mouse endometrial epithelial cells using reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and intracellular pH (pHi) measurement techniques. The presence of NHE1, NHE2, and NHE4, but not NHE3 mRNAs were revealed by RT-PCR. Immunostaining showed that NHE1, NHE2, and NHE4 were present in both apical and basolateral membranes. The pHi recovery from intracellular acidification was Na+-dependent; however, the rate of pHi recovery depending on basolateral Na+ was 12.4 times faster than that depending on apical Na+. The Na+-dependent rate of pHi recovery was also inhibited by amiloride, indicating H+ extrusion through NHEs; however, the amiloride sensitivity of the apical membrane was less than that of the basolateral membrane, suggesting the involvement of different types of NHEs in the two membranes. The results indicate that the basolaterally located NHE1, NHE2, and NHE4, in addition to participating in the homeostatic control of intracellular pH, may play a role in H+ extrusion in order to achieve transepithelial HCO3- secretion. The apically located NHEs may be involved in mediating Na+ absorption as alternatives of or complementary to epithelial Na+ channels.

1 This work was carried out at the Epithelial Cell Biology Research Center and was supported by a direct grant (204-775) from the strategic research program of The Chinese University of Hong Kong, and National 973 Project of China.

2 Correspondence: Hsiao Chang Chan, Department of Physiology, The Chinese University of Hong Kong, Shatin, Hong Kong. FAX: 852 2603 5022; hsiaocchan{at}cuhk.edu.hk






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Copyright © 2003 by the Society for the Study of Reproduction.