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Gene Expression in Mouse Oocytes and Preimplantation Embryos: Use of Suppression Subtractive Hybridization to Identify Oocyte- and Embryo-Specific Genes¹

^a Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6018

The paucity of biological material has inhibited identifying genes that are differentially expressed during mammalian oogenesis and preimplantation development. We report here the linear amplification of mRNA from small numbers of mouse oocytes and preimplantation embryos to generate amounts of sense RNA that are sufficient for suppression subtractive hybridization. The resulting oocyte-specific and 8-cell-specific cDNA libraries were partially characterized, and the known oocyte-specific ZP1, ZP2, GDF-9, BMP15, and H1_oo genes were found in the oocyte-specific cDNA library but not in the 8-cell-specific library. Further characterization of the subtracted oocyte and 8-cell embryo cDNA libraries should furnish a trove of information regarding temporal changes in gene expression during oogenesis and preimplantation development in the mouse.

¹ Supported by a grant from the NIH to R.M.S. (HD 22681). F.Z. was supported by a training grant from the NIH (T32 HD07516). Portions of this work are being submitted by F.Z. in partial fulfillment of the Ph.D. requirements at the University of Pennsylvania.

² Correspondence: Richard M. Schultz, Department of Biology, University of Pennsylvania, 415 South University Avenue, Philadelphia, PA 19104-6018. FAX: 215 898 8780; rschultz{at}mail.sas.upenn.edu

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