HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 68/2/699    most recent biolreprod.102.008540v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Weitzel, J. M. Articles by Seitz, H. J. Search for Related Content PubMed PubMed Citation Articles by Weitzel, J. M. Articles by Seitz, H. J. Agricola Articles by Weitzel, J. M. Articles by Seitz, H. J.

Testis-Specific Expression of Rat Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Haploid Male Germ Cells¹

^a Institute of Medical Biochemistry and Molecular Biology ^b Institute of Anatomy, University Hospital Hamburg-Eppendorf, D-20246 Hamburg, Germany ^c Institute for Hormone and Fertility Research, University of Hamburg, D-22529 Hamburg, Germany

Mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH) is regulated by multiple promoters in a tissue-specific manner. We characterized the testis-specific promoter C of the mGPDH gene and investigated the cellular localization of mGPDH within the testis. Electrophoretic mobility shift experiments identified a cAMP-response element (CRE) site at -57 that was active in the testis. An in vitro-translated CRE modulator (CREM) protein was able to bind this CRE site, and an anti-CREM antibody interfered with this complex. Ectopic expression of the testis-specific transcriptional activator CREM and protein kinase A in human hepatocarcinoma HepG2 cells activated a promoter C-driven luciferase construct in transient transfection experiments. Furthermore, mGPDH expression was undetectable in testis of CREM-deficient mice. The cellular localization of mGPDH expression and translation in adult rat testis was determined by in situ hybridization and immunohistochemistry techniques. The mGPDH transcripts were detected solely in postmeiotic germ cells. Expression of mGPDH was restricted from round spermatids to early elongating spermatids. The mGPDH protein was delayed in postmeiotic germ cells, restricted from late elongating spermatids to mature spermatids. Our results indicate that rat mGPDH is expressed by a testis-specific promoter from haploid male germ cells in a stage-specific manner.

¹ This work was supported by grants from the Deutscher Akademischer Austausch Dienst to N.B.S. and from the Deutsche Forschungsgemeinschaft (GRK336; FOR197/3-2) to J.M.W., R.M., R.I., and H.J.S. The nucleotide sequence described in this paper has been deposited in GenBank/EMBL under accession number AJ495842.

² Correspondence: Joachim M. Weitzel, Institute of Medical Biochemistry and Molecular Biology, University Hospital Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany. FAX: 49 40 42803 4862; weitzel{at}uke.uni-hamburg.de

This article has been cited by other articles:

				M. Rajkovic, R. Middendorff, M. G. Wetzel, D. Frkovic, S. Damerow, H. J. Seitz, and J. M. Weitzel Germ Cell Nuclear Factor Relieves cAMP-response Element Modulator {tau}-mediated Activation of the Testis-specific Promoter of Human Mitochondrial Glycerol-3-phosphate Dehydrogenase J. Biol. Chem., December 10, 2004; 279(50): 52493 - 52499. [Abstract] [Full Text] [PDF]