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Prenatal Exposure to Estrogenic Compounds Alters the Expression Pattern of Platelet-Derived Growth Factor Receptors and ß in Neonatal Rat Testis: Identification of Gonocytes as Targets of Estrogen Exposure¹

^a Division of Hormone Research, Department of Cell Biology, Georgetown University School of Medicine, Washington, District of Columbia 20057

We examined the effects of maternal exposure to estrogens on platelet-derived growth factor (PDGF) receptor (PDGFR) expression in newborn rat testis. Pregnant rats were treated from gestation Day 14 to birth with corn oil containing diethylstilbestrol, bisphenol A, genistein, or coumestrol by gavage or subcutaneous injection. These treatments induced a dose-dependent increase in the expression of PDGFR and ß mRNAs, determined by semiquantitative reverse transcription polymerase chain reaction, though diethylstilbestrol had a biphasic effect on both mRNAs. In situ hybridization analysis showed that PDGFR mRNA increased mostly in the interstitium, while PDGFRß mRNA increased both in the interstitium and seminiferous cords. Immunohistochemical studies of PDGFR and ß proteins revealed that both receptors were present in testis before and after birth and that they were upregulated upon treatment with estrogens in 3-day-old rats, with PDGFRß increasing dramatically in gonocytes. PDGFR and ß mRNAs and proteins were also found in purified gonocytes. Our previous finding that PDGF and 17ß-estradiol induce gonocyte proliferation in vitro, together with the present finding that in vivo exposure to estrogens upregulates PDGF receptors in testis, suggest that PDGF pathway is a target of estrogens in testis. In addition, these data identify PDGFRß in gonocytes as a major target of gestational estrogen exposure, suggesting that estrogen may have a physiological interaction with PDGF during gonocyte development. These results, however, do not exclude the possibility that the effects of the compounds examined in this study might be due to estrogen receptor-independent action(s).

¹ This research was supported by a grant from the NIEHS, award ES10366.

² Correspondence: Martine Culty, Department of Cell Biology, Georgetown University School of Medicine, 3900 Reservoir Road NW, Washington, DC 20057. FAX: 202 687 7855; e-mail: cultym{at}georgetown.edu

³ These authors equally contributed to this work

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