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This Article Full Text Full Text (PDF) All Versions of this Article: 68/3/961    most recent biolreprod.102.009480v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, Z. Articles by Short, R. V. Search for Related Content PubMed PubMed Citation Articles by Zhang, Z. Articles by Short, R. V. Agricola Articles by Zhang, Z. Articles by Short, R. V.

Successful Intra- and Interspecific Male Germ Cell Transplantation in the Rat¹

^a Departments of Zoology ^b Obstetrics and Gynaecology, University of Melbourne, Victoria 3010 Australia

The lumen of the seminiferous tubules has hitherto been regarded as an immunologically privileged site. We report here the birth of young following transplantation of stem spermatogonia from Long-Evans rats to the seminiferous tubules of Sprague-Dawley rats after treatment with the immunosuppressive agent cyclosporin. Follicle-stimulating hormone was also given to stimulate Sertoli cell proliferation, and testosterone to stimulate the recovery of spermatogenesis. Donor germ cells underwent normal spermatogenesis, and progeny were repeatedly produced from the donor germ cells as demonstrated by microsatellite paternity analysis. In addition, donor germ cells from the cryptorchid testes of LacZ mice were also able to colonize the seminiferous tubules of Sprague-Dawley rats using this protocol. Morphologically normal rat and mouse spermatozoa were present in the epididymis and vas deferens of the recipient rats. This highlights the potential for transplantation of male germ cells between different species.

¹ Supported by a grant from the Ramaciotti Foundation to R.V.S. and M.B.R., and by a Melbourne Research Scholarship to Z.Z.

² Correspondence. FAX: 61 3 9348 1719; e-mail: m.renfree{at}zoology.unimelb.edu.au

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