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Female Reproductive Tract |
a Institute of Molecular Animal Breeding, Ludwig Maximilians University, 81377 Munich, Germany
b Gene Center of the Ludwig Maximilians University, 81377 Munich, Germany
c Bavarian Research Center for Biology of Reproduction, 85764 Oberschleissheim, Germany
We studied differential gene expression in ipsilateral and contralateral bovine oviduct epithelial cells using a combination of subtracted cDNA libraries and cDNA array hybridization. Four Simmental heifers were synchronized and slaughtered 3.5 days after they entered standing heat. Epithelial cells were isolated from ipsilateral and contralateral oviducts. To identify genes that are differentially regulated in ipsilateral and contralateral epithelium, subtracted cDNA libraries were produced by suppression subtractive hybridization and analyzed by cDNA array hybridization. Sequencing of cDNAs showing differential expression levels in ipsilateral and contralateral epithelium revealed 35 different cDNAs, 30 of which matched genes with known functions and 5 of which matched genes without a known function. The majority of genes (n = 27) were expressed at a higher level in the ipsilateral oviduct, but for some genes (n = 8), mRNA abundance was higher in the contralateral oviduct. The regulated genes or their products include a variety of functional classes such as cell-surface proteins, cell-cell interaction proteins, members of signal transduction pathways, immune-related proteins, and enzymes. Identification of genes differentially regulated in ipsilateral and contralateral oviduct epithelial cells is the first step toward a systematic analysis of local mechanisms that regulate the function of the bovine oviduct epithelium in the postovulation period.
2 Correspondence: Eckhard Wolf, Institute of Molecular Animal Breeding, Ludwig Maximilians University, Feodor-Lynen-Str. 25, 81377 Munich, Germany. FAX: 49 89 218076849; ewolf{at}lmb.uni-muenchen.de
3 Present address: Institute of Physiology, Technical University Munich-Weihenstephan, Weihenstephaner Berg 3, 85350 Freising, Germany
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