Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


BOR - Papers in Press, published online ahead of print November 27, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.008912
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
68/4/1413    most recent
biolreprod.102.008912v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagaoka, K.
Right arrow Articles by Christenson, R. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagaoka, K.
Right arrow Articles by Christenson, R. K.
Agricola
Right arrow Articles by Nagaoka, K.
Right arrow Articles by Christenson, R. K.
BIOLOGY OF REPRODUCTION 68, 1413–1421 (2003)
DOI: 10.1095/biolreprod.102.008912
© 2003 by the Society for the Study of Reproduction, Inc.


Pregnancy

A Chemokine, Interferon (IFN)-{gamma}-Inducible Protein 10 kDa, Is Stimulated by IFN-{tau} and Recruits Immune Cells in the Ovine Endometrium1

Kentaro Nagaokaa, Akiharu Sakaia, Hisashi Nojimaa, Yoshihito Sudaa, Yuichi Yokomizob, Kazuhiko Imakawa2,a, Senkiti Sakaia, and Ronald K. Christensonc

a Laboratory of Animal Breeding, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan b National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan c Reproduction Research Unit, USDA-ARS, U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166

Proper distribution of immune cells in the uterus is a prerequisite for successful implantation and subsequent placentation, but biochemical signals that govern such events have not been well characterized. In the present study, the cDNA of a chemokine, interferon (IFN)-{gamma}-inducible protein 10 kDa (IP-10), was identified from a cDNA subtraction study between uterine endometrial tissues from Day 17 pregnant and Day 15 cyclic ewes. The effect of IFN-{tau} on IP-10 expression and the involvement of IP-10 in the recruitment of immune cells were then investigated. Northern blot analysis revealed that large amounts of IP-10 mRNA were present during conceptus attachment to maternal endometrium and early placentation. IP-10 mRNA was localized to monocytes distributed in the subepithelial stroma of pregnant but not cyclic uteri. This finding was supported by the discovery of IP-10 mRNA expression in monocytes but not in lymphocytes, uterine epithelial cells, or stromal cells. Moreover, the expression of IP-10 mRNA by the monocytes was stimulated by IFN-{alpha}, IFN-{gamma}, and IFN-{tau} in a dose-dependent manner, but the expression of IP-10 mRNA by the endometrial explants was most stimulated by IFN-{tau}. In a chemotaxis assay, migration of peripheral blood mononuclear cells was stimulated by the addition of IFN-{tau} stimulated-endometrial culture medium, and the effect was significantly reduced by neutralization with an anti-IP-10 antibody. These results suggest that endometrial IP-10 regulated by conceptus IFN-{tau} regulates recruitment and/or distribution of immune cells seen in the early pregnant uterus.

1 This work was supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan, by the Program for Promotion of Basic Research Activities for Innovative Bioscience (BRAIN), and by a Grant-in-Aid of Recombinant Cytokines Project from the Ministry of Agriculture, Forestry and Fisheries of Japan. K.N. was supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.

2 Correspondence: Kazuhiko Imakawa, Laboratory of Animal Breeding, Faculty of Agriculture, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. FAX: 81 3 58418180; akaz{at}mail.ecc.u-tokyo.ac.jp







HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Society for the Study of Reproduction.