Biol Reprod Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


BOR - Papers in Press, published online ahead of print November 27, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.010843
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
68/5/1545    most recent
biolreprod.102.010843v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow My Folders
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshiura, Y.
Right arrow Articles by Nagahama, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshiura, Y.
Right arrow Articles by Nagahama, Y.
Agricola
Right arrow Articles by Yoshiura, Y.
Right arrow Articles by Nagahama, Y.
BIOLOGY OF REPRODUCTION 68, 1545–1553 (2003)
DOI: 10.1095/biolreprod.102.010843
© 2003 by the Society for the Study of Reproduction, Inc.


Ovary

Synergistic Expression of Ad4BP/SF-1 and Cytochrome P-450 Aromatase (Ovarian Type) in the Ovary of Nile Tilapia, Oreochromis niloticus, During Vitellogenesis Suggests Transcriptional Interaction1

Yasutoshi Yoshiura3,4, Balasubramanian Senthilkumaran3,7, Masatada Watanabe3,5, Yuichi Oba3,6, Tohru Kobayashi3,7, and Yoshitaka Nagahama2,3,7

Laboratory of Reproductive Biology,3 National Institute for Basic Biology, Okazaki 444-8585, Japan Immunology Section,4 Inland Station, National Research Institute of Aquaculture, Tamaki, Mie 519-0423, Japan Department of Biochemistry,5 Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa-Ku, Tokyo 142-0063, Japan Graduate School of Bioagricultural Sciences,6 Nagoya University, Nagoya 464-8601, Japan CREST,7 Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan

Involvement of Ad4BP/SF-1 in the ovarian cytochrome P-450 aromatase (oP450arom) gene expression was investigated using ovarian follicles of the Nile tilapia, possessing an average 14-day spawning cycle. The promoter region (5' flanking region) of oP450arom gene cloned from tilapia contains two Ad4 binding sites. Subsequently, a cDNA encoding Ad4BP/SF-1 was cloned from the ovarian follicles. It is expressed in gonadal tissues, brain, and kidney. Oligonucleotide probes containing putative orphan nuclear receptor binding motifs (derived from promoter region of the aromatase gene) formed complexes with in vitro-translated Ad4BP/SF-1 and nuclear extracts of tilapia ovarian (midvitellogenic) follicles, indicating that Ad4BP/SF-1 is one of the transcriptional regulators for aromatase gene expression. Northern blot analysis revealed that the expression of both oP450arom and Ad4BP/SF-1 increased in parallel with ovarian growth from Day 0 to Day 5 after spawning and declined sharply from Day 8 to Day 11. On the day of spawning (Day 14), the expression of both correlates became undetectable. In vitro incubation of post vitellogenic full-grown immature follicles (corresponding to Day 11 after spawning) with hCG purged both oP450arom and Ad4BP/SF-1 messenger RNA transcripts at 18 h. Conversely, in vitro incubation of late vitellogenic follicles (corresponding to Day 8 after spawning) with hCG retained Ad4BP/SF-1 messenger RNA transcripts more or less steadily and up-regulated oP450arom. Ad4BP/SF-1 probably acts as a transcriptional modulator to implement the paradoxical actions of gonadotropins on oP450arom gene.

1 Supported in part by Grants-in-Aid for Research for the Future (JSPS-RFTF 96L100401) and Priority Areas (07283101) from the Japan Society for the Promotion of Science; for Scientific Research from the Ministry of Education, Science, Sports, and Culture, Japan; Bio-Design Program from the Ministry of Agriculture, Forestry, and Fisheries, Japan; and CREST of JST (Japan Science and Technology).

2 Correspondence: Yoshitaka Nagahama, National Institute for Basic Biology, Okazaki 444-8585, Japan. FAX: 81 564 55 7556; nagahama{at}nibb.ac.jp




This article has been cited by other articles:


Home page
Biol. Reprod.Home page
S. Ijiri, H. Kaneko, T. Kobayashi, D.-S. Wang, F. Sakai, B. Paul-Prasanth, M. Nakamura, and Y. Nagahama
Sexual Dimorphic Expression of Genes in Gonads During Early Differentiation of a Teleost Fish, the Nile Tilapia Oreochromis niloticus
Biol Reprod, February 1, 2008; 78(2): 333 - 341.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
G.-C. Wu, S. Tomy, and C.-F. Chang
The Expression of nr0b1 and nr5a4 During Gonad Development and Sex Change in Protandrous Black Porgy Fish, Acanthopagrus schlegeli
Biol Reprod, February 1, 2008; 78(2): 200 - 210.
[Abstract] [Full Text] [PDF]


Home page
Mol. Endocrinol.Home page
D.-S. Wang, T. Kobayashi, L.-Y. Zhou, B. Paul-Prasanth, S. Ijiri, F. Sakai, K. Okubo, K.-i. Morohashi, and Y. Nagahama
Foxl2 Up-Regulates Aromatase Gene Transcription in a Female-Specific Manner by Binding to the Promoter as Well as Interacting with Ad4 Binding Protein/Steroidogenic Factor 1
Mol. Endocrinol., March 1, 2007; 21(3): 712 - 725.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
T.-T. Wong, S. Ijiri, and Y. Zohar
Molecular Biology of Ovarian Aromatase in Sex Reversal: Complementary DNA and 5'-Flanking Region Isolation and Differential Expression of Ovarian Aromatase in the Gilthead Seabream (Sparus aurata)
Biol Reprod, May 1, 2006; 74(5): 857 - 864.
[Abstract] [Full Text] [PDF]


Home page
J Mol EndocrinolHome page
S Kuntz, D Chardard, C-I Ko, H Dumond, M Ducatez, M Callier, S Flament, and A Chesnel
Female-enriched and thermosensitive expression of steroidogenic factor-1 during gonadal differentiation in Pleurodeles waltl
J. Mol. Endocrinol., February 1, 2006; 36(1): 175 - 186.
[Abstract] [Full Text] [PDF]


Home page
J Mol EndocrinolHome page
L Y Zhou, D S Wang, B Senthilkumaran, M Yoshikuni, Y Shibata, T Kobayashi, C C Sudhakumari, and Y Nagahama
Cloning, expression and characterization of three types of 17{beta}-hydroxysteroid dehydrogenases from the Nile tilapia, Oreochromis niloticus
J. Mol. Endocrinol., August 1, 2005; 35(1): 103 - 116.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Society for the Study of Reproduction.