HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 68/5/1562    most recent biolreprod.102.008219v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kagawa, H. Articles by Tanaka, H. Search for Related Content PubMed PubMed Citation Articles by Kagawa, H. Articles by Tanaka, H. Agricola Articles by Kagawa, H. Articles by Tanaka, H.

Effects of Luteinizing Hormone and Follicle-Stimulating Hormone and Insulin-Like Growth Factor-I on Aromatase Activity and P450 Aromatase Gene Expression in the Ovarian Follicles of Red Seabream, Pagrus major¹

National Research Institute of Aquaculture,³ Fisheries Research Agency, Nansei, Mie 516-0193, Japan Tamaki Inland Station,⁴ National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, Mie 519-0423, Japan

To clarify the mechanism of estradiol-17ß production in the ovarian follicle of red seabream, in vitro effects of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and insulin-like growth factor (IGF-I) on aromatase activity (conversion of testosterone to estradiol-17ß) and cytochrome P450 aromatase (P450arom) mRNA expression in ovarian fragments of red seabream were investigated. Of the growth factors used in the present study, only IGF-I stimulated both aromatase activity and P450arom gene expression in the ovarian fragments of red seabream. LH from red seabream pituitary, but not FSH, stimulated both aromatase activity and P450arom gene expression. IGF-I slightly enhanced the LH-induced aromatase activity and P450arom gene expression. These data and our previous results indicate that LH, but not FSH, stimulates estradiol-17ß production in the ovarian follicle of red seabream through stimulation of aromatase activity and P450arom gene expression and IGF-I enhances the LH-stimulated P450arom gene expression.

¹ Supported in part by a grant-in-aid from the Ministry of Agriculture, Forestry, and Fisheries, Japan (Bio-Design Program).

² Correspondence. FAX: 81 599 66 1962; hkagawa{at}fra.affrc.go.jp

This article has been cited by other articles:

				S. Paul, D. Mukherjee, K. Pramanick, S. Kundu, S P Bhattacharyya, P. De, and D. Mukherjee Stimulation of salmon calcitonin on secretion of 17{beta}-estradiol by the ovarian follicles of common carp, Cyprinus carpio J. Endocrinol., February 1, 2008; 196(2): 413 - 424. [Abstract] [Full Text] [PDF]

				H. Ko, W. Park, D.-J. Kim, M. Kobayashi, and Y. C. Sohn Biological activities of recombinant Manchurian trout FSH and LH: their receptor specificity, steroidogenic and vitellogenic potencies J. Mol. Endocrinol., January 1, 2007; 38(1): 99 - 111. [Abstract] [Full Text] [PDF]

				B. Campbell, J. Dickey, B. Beckman, G. Young, A. Pierce, H. Fukada, and P. Swanson Previtellogenic Oocyte Growth in Salmon: Relationships among Body Growth, Plasma Insulin-Like Growth Factor-1, Estradiol-17beta, Follicle-Stimulating Hormone and Expression of Ovarian Genes for Insulin-Like Growth Factors, Steroidogenic-Acute Regulatory Protein and Receptors for Gonadotropins, Growth Hormone, and Somatolactin Biol Reprod, July 1, 2006; 75(1): 34 - 44. [Abstract] [Full Text] [PDF]

				T.-T. Wong, S. Ijiri, and Y. Zohar Molecular Biology of Ovarian Aromatase in Sex Reversal: Complementary DNA and 5'-Flanking Region Isolation and Differential Expression of Ovarian Aromatase in the Gilthead Seabream (Sparus aurata) Biol Reprod, May 1, 2006; 74(5): 857 - 864. [Abstract] [Full Text] [PDF]