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Ovarian Modulators of 11ß-Hydroxysteroid Dehydrogenase (11ßHSD) Activity in Follicular Fluid from Bovine and Porcine Large Antral Follicles and Spontaneous Ovarian Cysts¹

Department of Veterinary Basic Sciences,³ Royal Veterinary College, Camden Town, London NW1 0TU, United Kingdom Department of Biochemistry & Molecular Biology,⁴ Royal Free & University College Medical School, University College London, London NW3 2PF, United Kingdom

In the ovary, cortisol is oxidized to cortisone by 11ß-hydroxysteroid dehydrogenase (11ßHSD). The present study investigated whether follicular fluid (FF) from large antral follicles and spontaneous ovarian cysts, isolated from bovine and porcine ovaries, contained modulators of 11ßHSD activity. Whereas FF from antral follicles had no significant effect over 1 h on NADP⁺-dependent 11ßHSD activity in rat kidney homogenates, enzyme activity was inhibited by FF from bovine and porcine ovarian cysts (80.5% ± 2.3% and 72.8% ± 3.4% of control, respectively). Following C18 reverse-phase chromatography, the hydrophilic fractions of FF from bovine and porcine antral follicles stimulated NADP⁺-dependent 11ßHSD activities (111.5% ± 21.6% and 55.2% ± 5.7% respectively). Hydrophobic compounds inhibited NADP⁺-dependent cortisol oxidation by 58.2% ± 5.1% (bovine) and 45.7% ± 2.0% (porcine). In both species, FF from ovarian cysts appeared to contain less of the hydrophilic stimuli to 11ßHSD activity and more of the hydrophobic inhibitors. The FF from antral follicles and ovarian cysts, and the C18 fractions thereof, had no significant effect on NAD⁺-dependent cortisol oxidation. The ovarian modulators of NADP⁺-dependent 11ßHSD activities did not coelute with cortisol, cortisone, estradiol, testosterone, progesterone, pregnenolone, and cholesterol. However, the 11ßHSD stimuli in porcine FF from both antral follicles and cysts coeluted with prostaglandin (PG) E₂ and PGF₂. We conclude that large antral follicles and spontaneous ovarian cysts, in both the cow and the pig, contain ovarian modulators of the NADP⁺-dependent 11ßHSD activity. Moreover, FF from spontaneous ovarian cysts, because of decreased content of the 11ßHSD stimulus accompanied by increased content of the 11ßHSD inhibitors, exerts a net inhibitory effect on 11ßHSD activity.

¹ Supported by a grant from Freemedic plc (London, U.K.), BBSRC project grant 48/S15850, and MRC Studentship G69/1756.

² Correspondence: Lisa Thurston, Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, Camden Town, London NW1 0TU, United Kingdom. FAX: 44 0 20 7388 1027; lthurston{at}rvc.ac.uk

This article has been cited by other articles:

				N. Sunak, D. F Green, L. R Abeydeera, L. M Thurston, and A. E Michael Implication of cortisol and 11{beta}-hydroxysteroid dehydrogenase enzymes in the development of porcine (Sus scrofa domestica) ovarian follicles and cysts Reproduction, June 1, 2007; 133(6): 1149 - 1158. [Abstract] [Full Text] [PDF]