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CPEB2, A Novel Putative Translational Regulator in Mouse Haploid Germ Cells¹

Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences,³ and Department of Chemistry and Bioengineering, Graduate School of Engineering,⁴ Yokohama National University, Yokohama 240-8501, Japan Laboratory of Cytogenetics, Division of Bioscience, Graduate School of Environmental Earth Science,⁵ and Chromosome Research Unit, Faculty of Science,⁶ Hokkaido University, Sapporo 060-0810, Japan Department of Genetic Information,⁷ School of Medicine, Tokai University, Isehara 259-1153, Japan Department of Obstetrics and Gynecology, Center for Research on Reproduction and Women's Health,⁸ School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6142

Translational control of specific mRNAs by cytoplasmic polyadenylation has fundamental roles in gametogenesis. The cytoplasmic polyadenylation element binding (CPEB) protein regulates cytoplasmic polyadenylation of mRNAs as a trans factor in oogenesis and spermatogenesis. The CPEB protein contains two RNA recognition motifs and a Zn-finger structure. Proteins (KIAA0940 and KIAA1673) with similar structures are predicted from the genome database, but nothing is known about their expression and function. Here, we report another novel member of the CPEB protein family, CPEB2. Comparison of the amino acid sequences of CPEB family members suggests that the family can be divided structurally and, perhaps, functionally into two groups: the CPEB group, and the CPEB2-KIAA0940-KIAA1673 group. The CPEB2 maps to mouse chromosome distal 5B and is abundantly expressed in testis. However, it was detected by reverse transcription-polymerase chain reaction in all tissues that we examined. It preferentially binds to poly(U) and localizes to the cytoplasm in transfected HeLa cells. The CPEB2 is expressed postmeiotically in mouse spermatogenesis, suggesting a possible role in translational regulation of stored mRNAs in transcriptionally inactive haploid spermatids.

¹ Supported by grants from Ministry of Education, Science, Sports and Culture of Japan, Kanagawa Academy of Science and Technology Research, and Kihara Memorial Yokohama Foundation.

² Correspondence: Yasuyuki Kurihara, Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, Tokiwa-dai, Hodogaya, Yokohama 240-8501, Japan. FAX: 81 45 339 4263; kurihara{at}mac.bio.bsk.ynu.ac.jp