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BOR - Papers in Press, published online ahead of print April 2, 2003.
Biol Reprod 2003, 10.1095/biolreprod.102.011791
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BIOLOGY OF REPRODUCTION 69, 310–321 (2003)
DOI: 10.1095/biolreprod.102.011791
© 2003 by the Society for the Study of Reproduction, Inc.

Testis

{gamma}-Tubulin Overexpression in Sertoli Cells In Vivo: I. Localization to Sites of Spermatid Head Attachment and Alterations in Sertoli Cell Microtubule Distribution1

Shawna L. Fleming3, Peter R. Shank4, and Kim Boekelheide2,3

Departments of Pathology and Laboratory Medicine3 Molelcular Microbiology and Immunology,4 Brown University, Providence, Rhode Island 02912

Sertoli cells play a number of roles in supporting spermatogenesis, including structural organization, physical and paracrine support of germ cells, and secretion of factors necessary for germ cell development. Studies with microtubule disrupting compounds indicate that intact microtubule networks are crucial for normal spermatogenesis. However, treatment with toxicants and pharmacologic agents that target microtubules lack cell-type selectivity and may therefore elicit direct effects on germ cells, which also require microtubule-mediated activities for division and morphological transformation. To evaluate the importance of Sertoli cell microtubule-based activities for spermatogenesis, an adenoviral vector that overexpresses the microtubule nucleating protein, {gamma}-tubulin, was used to selectively disrupt microtubule networks in Sertoli cells in vivo. {gamma}-Tubulin overexpression was observed to cause redistribution of Sertoli cell microtubule networks, and overexpression of a {gamma}-tubulin-enhanced green fluorescent protein fusion protein was observed to localize to the site of elongate spermatid head attachment to the seminiferous epithelium.

1 This work was funded in part by RO1ES08956 from the National Institutes of Environmental Health Sciences.

2 Correspondence. Kim Boekelheide, Department of Pathology and Laboratory Medicine, Brown University, Providence, Rhode Island 02912. FAX: 401 863 9008; Kim_Boekelheide{at}Brown.edu




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