| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reproductive Technology |
The Fels Institute for Cancer Research and Molecular Biology3
Department of Biochemistry,4 Temple University School of Medicine, Philadelphia, Pennsylvania 19140
Division of Reproductive Endocrinology and Infertility,5 Washington University School of Medicine, St. Louis, Missouri 63130
Cloning by somatic cell nuclear transfer requires silencing of the donor cell gene expression program and the initiation of the embryonic gene expression program (nuclear reprogramming). Failure to silence the donor cell program could lead to altered embryonic phenotypes. Cloned mouse embryos produced using myoblast nuclei fail to thrive in standard embryo culture media but flourish in somatic cell culture media favored by the donor myoblasts themselves, forming blastocysts at a significant rate, with robust morphologies, high total cell number, and a normal allocation of cells to the inner cell mass in most embryos. Myoblast cloned embryos continue expressing the GLUT4 glucose transporter, which is typically expressed in muscle but not in preimplantation stage embryos. Myoblast clones also exhibit precocious enrichment of GLUT1 at the cell surface. Both myoblast and cumulus cell cloned embryos exhibit enhanced rates of glucose uptake. These observations indicate that silencing of the donor cell genome during cloning either is incomplete or occurs progressively over the course of preimplantation development. As a result, cloned embryos initially exhibit many somatic cell-like characteristics. Tetraploid constructs, which possess a transplanted somatic cell genome plus the oocyte-derived chromosomes, exhibit a more embryonic-like pattern of gene expression and culture preference. We conclude that preimplantation stage cloned embryos have profoundly altered characteristics that are donor cell type specific and that exposure of cloned embryos to standard embryo culture conditions may lead to disruptions in basic homeostasis and inhibition of a range of essential processes including further nuclear reprogramming, contributing to cloned embryo demise.
2 Correspondence. FAX: 215-707-1454; klatham{at}unix.temple.edu
This article has been cited by other articles:
|
|
 |
|
  V. Duranthon, A. J Watson, and P. Lonergan Preimplantation embryo programming: transcription, epigenetics, and culture environment Reproduction, February 1, 2008; 135(2): 141 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. R Mtango and K. E Latham Differential Expression of Cell Cycle Genes in Rhesus Monkey Oocytes and Embryos of Different Developmental Potentials Biol Reprod, February 1, 2008; 78(2): 254 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Wang, Z. Kou, Y. Zhang, and S. Gao Dynamic Reprogramming of Histone Acetylation and Methylation in the First Cell Cycle of Cloned Mouse Embryos Biol Reprod, December 1, 2007; 77(6): 1007 - 1016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.L Green, D.N Wells, and B Oback Cattle Cloned from Increasingly Differentiated Muscle Cells Biol Reprod, September 1, 2007; 77(3): 395 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K Berg, C. Li, G. Asher, D. N Wells, and B. Oback Red Deer Cloned from Antler Stem Cells and Their Differentiated Progeny Biol Reprod, September 1, 2007; 77(3): 384 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Heindryckx, P. De Sutter, J. Gerris, M. Dhont, and J. Van der Elst Embryo development after successful somatic cell nuclear transfer to in vitro matured human germinal vesicle oocytes Hum. Reprod., July 1, 2007; 22(7): 1982 - 1990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Smith, D. Berg, S. Beaumont, N. T Standley, D. N Wells, and P. L Pfeffer Simultaneous gene quantitation of multiple genes in individual bovine nuclear transfer blastocysts Reproduction, January 1, 2007; 133(1): 231 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Somers, C. Smith, M. Donnison, D. N Wells, H. Henderson, L. McLeay, and P L Pfeffer Gene expression profiling of individual bovine nuclear transfer blastocysts. Reproduction, June 1, 2006; 131(6): 1073 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Inoue, N. Ogonuki, H. Miki, M. Hirose, S. Noda, J.-M. Kim, F. Aoki, H. Miyoshi, and A. Ogura Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer J. Cell Sci., May 15, 2006; 119(10): 1985 - 1991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Armstrong, M. Lako, W. Dean, and M. Stojkovic Epigenetic Modification Is Central to Genome Reprogramming in Somatic Cell Nuclear Transfer Stem Cells, April 1, 2006; 24(4): 805 - 814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Brambrink, K. Hochedlinger, G. Bell, and R. Jaenisch ES cells derived from cloned and fertilized blastocysts are transcriptionally and functionally indistinguishable PNAS, January 24, 2006; 103(4): 933 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yu, J. Yong, X. Li, T. Qing, H. Qin, X. Xiong, J. You, M. Ding, and H. Deng The proteasomal inhibitor MG132 increases the efficiency of mouse embryo production after cloning by electrofusion Reproduction, October 1, 2005; 130(4): 553 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takeda, M. Tasai, M. Iwamoto, A. Onishi, T. Tagami, K. Nirasawa, H. Hanada, and C. A. Pinkert Microinjection of Cytoplasm or Mitochondria Derived from Somatic Cells Affects Parthenogenetic Development of Murine Oocytes Biol Reprod, June 1, 2005; 72(6): 1397 - 1404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Eckardt, N A. Leu, S. Kurosaka, and K J. McLaughlin Differential reprogramming of somatic cell nuclei after transfer into mouse cleavage stage blastomeres Reproduction, May 1, 2005; 129(5): 547 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. D. Morgan, F. Santos, K. Green, W. Dean, and W. Reik Epigenetic reprogramming in mammals Hum. Mol. Genet., April 15, 2005; 14(suppl_1): R47 - R58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.P. Enright, L.-Y. Sung, C.-C. Chang, X. Yang, and X.C. Tian Methylation and Acetylation Characteristics of Cloned Bovine Embryos from Donor Cells Treated with 5-aza-2'-Deoxycytidine Biol Reprod, April 1, 2005; 72(4): 944 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.M. Powell, N.C. Talbot, K.D. Wells, D.E. Kerr, V.G. Pursel, and R.J. Wall Cell Donor Influences Success of Producing Cattle by Somatic Cell Nuclear Transfer Biol Reprod, July 1, 2004; 71(1): 210 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gao, E. Czirr, Y. G. Chung, Z. Han, and K. E. Latham Genetic Variation in Oocyte Phenotype Revealed Through Parthenogenesis and Cloning: Correlation with Differences in Pronuclear Epigenetic Modification Biol Reprod, April 1, 2004; 70(4): 1162 - 1170. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
