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Mechanisms of Hormone Action |
Department of Aquaculture,3 National Taiwan Ocean University, Keelung 202, Taiwan, Republic of China
National Museum of Marine Biology and Aquarium,4 Pintung 944, Taiwan, Republic of China
Laboratory of Reproductive Biology,5 National Institute for Basic Biology, Okazaki 444, Japan
Black porgy, Acanthopagrus schlegeli Bleeker, is a marine protandrous hermaphrodite fish. All are functional males at 12 yr of age and then become either males or females at 3 yr of age. To study the process of sex change in this species, mRNA transcripts of two estrogen receptors (ER
and ERß) and an androgen receptor (AR) were monitored. An AR cDNA was cloned and characterized. ER
, ERß, and AR were differentially transcribed in bisexual testicular and ovarian tissue according to reverse transcription polymerase chain reaction (RT-PCR) and Southern analysis. A real-time quantification PCR analysis was further developed for the measurement of AR, ER
, and ERß transcripts. ER
and AR transcripts were significantly more plentiful in bisexual testis than in bisexual ovary in 1+- and 2+-yr-old fish. ER
, ERß, and AR transcripts decreased in the functional testis of 3-yr-old fish. Similar levels of ERß and AR were detected in the ovary of sex-changed females and in functional testis of 3-yr-old males. Significantly decreased AR transcripts were found in testicular tissue of bisexual and functional male and female gonads in 3-yr-old fish as compared with 1- and 2-yr-old fish. In contrast, increased ER
transcripts were detected in the bisexual ovary and sex-changed ovary of 3-yr-old fish as compared with the bisexual ovary of 1- and 2-yr-old fish. The data suggest a differential sensitivity in the bisexual testicular and ovarian tissue of black porgy.
2 Correspondence: Ching-Fong Chang, Department of Aquaculture, National Taiwan Ocean University, Keelung 202, Taiwan. FAX: 886 2 2462 1579; b0044{at}mail.ntou.edu.tw
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