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BOR - Papers in Press, published online ahead of print April 30, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.017590
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BIOLOGY OF REPRODUCTION 69, 695–700 (2003)
DOI: 10.1095/biolreprod.103.017590
© 2003 by the Society for the Study of Reproduction, Inc.

Ovary

Effects of Prostaglandin F2{alpha} and Progesterone on the Ability of Bovine Luteal Cells to Stimulate T Lymphocyte Proliferation1

Matthew J. Cannon, Margaret G. Petroff3, and Joy L. Pate2

Department of Animal Sciences, The Ohio State University/Ohio Agricultural Research and Development Center, Wooster, Ohio 44691

Bovine luteal cells express class I and II major histocompatibility complex molecules and stimulate T lymphocyte proliferation in vitro. Proliferation of T lymphocytes is greater in cocultures of luteal cells and T lymphocytes collected following administration of a luteolytic dose of prostaglandin (PG) F2{alpha} to the cow. Whether this results from changes in luteal cells that increase their ability to stimulate T lymphocyte proliferation or from changes in T lymphocytes that enhance their ability to respond to luteal cells is unclear. To determine which is the case, luteal cell-T lymphocyte cocultures were performed using luteal cells and T lymphocytes isolated from the same animals before and 8 h after administration of PGF2{alpha}. In the presence of T lymphocytes collected before PGF2{alpha} administration, luteal cells isolated after PGF2{alpha} were more potent stimulators of T lymphocyte proliferation than were luteal cells collected before PGF2{alpha} (P < 0.05). The effect of progesterone on luteal cell-stimulated T lymphocyte proliferation was also evaluated. Proliferation of T lymphocytes was greater (P < 0.05) in cultures containing the cytochrome P450 side-chain cleavage enzyme-inhibitor aminoglutethimide. Exogenous progesterone caused a dose-dependent inhibition of luteal cell-stimulated T lymphocyte proliferation (P < 0.05). Progesterone-receptor mRNA was undetectable in peripheral blood mononuclear cells collected before and after PGF2{alpha} administration, indicating that the effect of progesterone was not mediated via progesterone receptors in lymphocytes. These results imply that specific changes in luteal cells in response to PGF2{alpha} enhance the ability of these cells to stimulate T lymphocyte proliferation. These results also demonstrate that progesterone can suppress luteal cell-stimulated T lymphocyte proliferation.

1 Supported in part by NIH grant HD37550; salaries and research support also provided by state and federal funds appropriated.

2 Correspondence: Joy L. Pate, Department of Animal Sciences, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691. FAX: 330 263 3949; pate.1{at}osu.edu

3 Current address: Anatomy and Cell Biology Unit, The University of Kansas Medical Center, 3901 Rainbow Blvd., Kansas City, KS 66160




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