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This Article Full Text Full Text (PDF) All Versions of this Article: 69/3/1002    most recent biolreprod.102.015065v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Glover, M. D. Articles by Seidel, G. E. Search for Related Content PubMed PubMed Citation Articles by Glover, M. D. Articles by Seidel, G. E., Jr Agricola Articles by Glover, M. D. Articles by Seidel, G. E.

Increased Messenger RNA for Allograft Inflammatory Factor-1, LERK-5, and a Novel Gene in 17.5-Day Relative to 15.5-Day Bovine Embryos¹

Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, Colorado 80523

Considerable embryonic loss occurs between Gestation Days 15 and 18 in cattle when critical cellular and molecular events occur, including maternal recognition of pregnancy. To gain insight into these events, mRNA differential display analysis was used to identify eight unique cDNA fragments present in greater abundance in 17.5-day than in 15.5-day bovine embryos. Four cDNA fragments, confirmed to be upregulated in 17.5-day embryos using Northern analysis, were cloned and sequenced. Three cDNA fragments shared sequence identities with known homologs: human allograft inflammatory factor-1 (AIF-1), human LERK-5, and bovine interferon-. One novel cDNA fragment did not share sequence identity to previously reported genes, except for a similar DNA sequence in the human genome. AIF-1 mRNA was present in developing placenta through Gestation Day 36, and abundant levels were observed in adult bovine spleen and lung. The novel gene, which we have named periattachment factor (PAF), was not detected in adult tissues using Northern analysis or in conceptuses between Days 30 and 36 of pregnancy. Additional sequence information for bPAF was obtained from a cDNA library constructed from a 25-day bovine embryo. The protein corresponding to the open reading frame has four protein kinase C phosphorylation sites, two casein kinase II phosphorylation sites, a nuclear targeting sequence, but no obvious DNA or RNA binding motifs. Abundant expression of this gene during a narrow but critical window of embryonic development makes it worthy of further study.

¹ This work was supported by NIH Grant R01-HD-27938 and the Colorado State University Experiment Station through Regional Project W-171. M.D.G. was supported in part by a Colorado Institute for Research in Biotechnology Fellowship.

² Correspondence: G.E. Seidel, Jr., ARBL, Colorado State University, Fort Collins, CO 80523. FAX: 970 491 3557; gseidel{at}colostate.edu

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