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This Article Full Text Full Text (PDF) All Versions of this Article: 69/3/797    most recent biolreprod.103.016899v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by O'Shaughnessy, P.J. Articles by Johnston, H. Search for Related Content PubMed PubMed Citation Articles by O'Shaughnessy, P.J. Articles by Johnston, H. Agricola Articles by O'Shaughnessy, P.J. Articles by Johnston, H.

Identification of Developmentally Regulated Genes in the Somatic Cells of the Mouse Testis Using Serial Analysis of Gene Expression¹

Institute of Comparative Medicine, University of Glasgow Veterinary School, Glasgow, United Kingdom

To identify genes developmentally regulated in the somatic cells of the testis, serial analysis of gene expression (SAGE) has been used to generate gene expression profiles from these cells in the fetal and adult mouse. To avoid germ cell transcripts, a fetal SAGE library was generated from germ cell-free fetal W^v/W^v mice, and an adult SAGE library was generated from adult testes depleted of germ cells with busulfan. The combined SAGE libraries contained 147 570 tags identifying 12 976 unique transcripts. Of these transcripts, 3607 were present in only the fetal library and 3941 were present in only the adult library. Most of the abundant differentially expressed tags in the adult testis library were from characterized genes, whereas 3' rapid amplification of complementary ends was required to identify most differentially expressed tags in the fetal library. These fetal tags were mostly associated with uncharacterized UniGene clusters. These data provide a comprehensive and quantitative analysis of gene expression in the somatic cells of the fetal and adult testis (including unknown transcripts) and identify genes differentially expressed in these cells during testis development. These differentially regulated genes are likely to provide insight into mechanisms regulating testis function both during development and in the adult animal.

¹ This study was supported by the Wellcome Trust and the Biotechnology and Biological Sciences Research Council.

² Correspondence: P.J. O'Shaughnessy, Institute of Comparative Medicine, University of Glasgow Veterinary School, Bearsden Rd, Glasgow G61 1QH, UK. FAX: 44 141 330 5797; p.j.oshaughnessy{at}vet.gla.ac.uk

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