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This Article Full Text Full Text (PDF) All Versions of this Article: 69/3/828    most recent biolreprod.103.016444v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McCauley, T.C. Articles by Day, B.N. Search for Related Content PubMed PubMed Citation Articles by McCauley, T.C. Articles by Day, B.N. Agricola Articles by McCauley, T.C. Articles by Day, B.N.

Oviduct-Specific Glycoprotein Modulates Sperm-Zona Binding and Improves Efficiency of Porcine Fertilization In Vitro¹

Department of Animal Sciences,⁴ University of Missouri, Columbia, Missouri 65211 Department of Obstetrics and Gynecology,⁵ College of Medicine, University of Florida, Gainesville, Florida 32610 Reproductive Genomic Program,⁶ Monsanto, St. Louis, Missouri 63017

Oviduct-specific glycoprotein (OGP) displays estrus-associated regional and temporal differences in expression and localizes to the zona pellucida, perivitelline space, and plasma membrane of oviductal oocytes and embryos, suggesting that it may have a role in regulation of fertilization and/or early embryonic development. The aims of this study were to evaluate the effect of exogenous OGP on in vitro fertilization (IVF) and embryo development in the pig using a defined serum-free culture system. In vitro-matured porcine oocytes were incubated with homologous OGP (0, 1, 10, 20, and 40 µg/ml) for 3 h and then washed prior to IVF. Exposure of oocytes to 10 or 20 µg/ml porcine OGP (pOGP) significantly reduced the incidence of polyspermy compared with the control (P < 0.01) while maintaining high penetration rates. When oocytes, spermatozoa, or both were preincubated with 10 µg/ml pOGP prior to IVF, the incidence of polyspermy was similarly reduced (P < 0.01) by all three treatments without affecting penetration rates. The ability of spermatozoa to undergo calcium ionophore-induced acrosome reaction was similar with or without exposure to pOGP. However, significantly fewer spermatozoa (P < 0.01) bound to the zona pellucida when oocytes were preincubated with pOGP. To evaluate the effect of pOGP on embryo development, embryos were cultured in pOGP-supplemented medium for 48 h or 144 h. Both transient and continuous exposure to pOGP significantly enhanced cleavage and blastocyst formation rate compared with the control (P < 0.01). These data demonstrate that exposure of either in vitro-matured oocytes or spermatozoa to pOGP decreased polyspermy and spermatozoa binding while maintaining high penetration rates of pig oocytes fertilized in vitro. Furthermore, pOGP exerted an embryotrophic effect independent of effects demonstrated on spermatozoa and oocytes at fertilization.

¹ Supported by Monsanto Corp.(St. Louis, MO).

² Correspondence: Billy N. Day, Department of Animal Sciences, 159 Animal Science Research Center, University of Missouri, Columbia, Missouri 65211. FAX: 573 884 7827; dayb{at}missouri.edu

³ Current address: TMI Laboratories, 850 N. Kolb Rd., Tucson, Arizona 85710

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