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BOR - Papers in Press, published online ahead of print May 28, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.015446
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BIOLOGY OF REPRODUCTION 69, 947–958 (2003)
DOI: 10.1095/biolreprod.103.015446
© 2003 by the Society for the Study of Reproduction, Inc.

Ovary

Determination of Steroidogenic Potential of Ovarian Cells of the Brushtail Possum (Trichosurus vulpecula)1

Lisa J. Whale, Douglas C. Eckery, and Jennifer L. Juengel2

AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand

The ovary of the brushtail possum (Trichosurus vulpecula) secretes steroids; however, little is known about the identity of the steroidogenic cells in the ovary. The aim of the present study was to determine the identity of the ovarian cell types expressing mRNAs encoding proteins important for steroidogenesis and determine at what stage of follicular development they are expressed. The genes examined were those for steroidogenic factor-1 (SF-1), steroidogenic acute regulatory protein (StAR), cytochrome P450 side chain cleavage (P450scc), 3ß-hydroxysteroid dehydrogenase/{Delta}5,{Delta}4 isomerase (3ßHSD), cytochrome P45017{alpha}hydroxylase (P45017{alpha}OH), and P450 aromatase (P450arom). None of the genes examined were expressed in oocytes at any stage of follicular development. SF-1 was expressed in granulosa cells from the type 2 or the primary stage of development and thereafter to the preovulatory stage. In addition, the theca interna of small and medium-size antral but not preovulatory follicles and the interstitial glands and corpora lutea expressed SF-1 mRNA. Granulosa cells of preantral and small to medium-size antral follicles were not capable of synthesizing steroids from cholesterol because they did not contain P450scc mRNA. However, granulosa cells of many of the small to medium-size antral follicles expressed P450arom and 3ßHSD mRNA. The interstitial glands, theca interna, and corpus luteum expressed StAR, P450scc, 3ßHSD, and P45017{alpha}OH mRNA, suggesting that these tissues are capable of synthesizing progestins and androgens. The corpus luteum expressed P450arom, indicating that this tissue also has the potential to secrete estrogens in this species.

1 This research was supported by the New Zealand Foundation for Research, Science and Technology.

2 Correspondence: Jennifer Juengel, Wallaceville Animal Research Centre, Ward St., P.O. Box 40063, Upper Hutt, New Zealand. FAX: 64 4 922 1380; jenny.juengel{at}agresearch.co.nz




This article has been cited by other articles:


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 J EndocrinolHome page
J L Crawford, B P Thomson, M F Beaumont, and D C Eckery
Plasma concentrations of prolactin in brushtail possums (Trichosurus vulpecula) in different physiological states.
J. Endocrinol., August 1, 2006; 190(2): 295 - 305.
[Abstract] [Full Text] [PDF]


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Copyright © 2003 by the Society for the Study of Reproduction.