| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Embryo |
Department of Anatomy and Physiology,2 Royal Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark
Department of Farm Animal Health,3 Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands
Department of Clinical Studies, Reproduction,4 Royal Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark
The postimplantation developmental potential of embryos can be affected by various forms of cell death, such as apoptosis, at preimplantation stages. However, correct assessment of apoptosis is needed for adequate inference of the developmental significance of this process. This study is the first to investigate the independent chronological occurrence of apoptotic changes in nuclear morphology and DNA degradation (detected by the TUNEL reaction) and incidences of nuclei displaying these features at various preimplantation stages of bovine embryos produced both in vivo and in vitro. Different elements of apoptosis were observed at various developmental stages and appeared to be differentially affected by in vitro production. Nuclear condensation was observed from the 6-cell stage in vitro and the 8-cell stage in vivo, whereas the TUNEL reaction was first observed at the 6-cell stage in vitro and the 21-cell stage in vivo. Morphological signs of other forms of cell death were also observed in normally developing embryos produced both in vivo and in vitro. The onset of apoptosis seems to be developmentally regulated in a stage-specific manner, but discrete features of the apoptotic process may be differentially regulated and independently modulated by the mode of embryo production. Significant differences in indices of various apoptotic features were not evident between in vivo- and in vitro-produced embryos at the morula stage, but such differences could be observed at the blastocyst stage, where in vitro production was associated with a higher degree of apoptosis in the inner cell mass.
This article has been cited by other articles:
|
|
 |
|
  N. I Alexopoulos, P. Maddox-Hyttel, P. Tveden-Nyborg, N. T D'Cruz, T. R Tecirlioglu, M. A Cooney, K. Schauser, M. K Holland, and A. J French Developmental disparity between in vitro-produced and somatic cell nuclear transfer bovine days 14 and 21 embryos: implications for embryonic loss Reproduction, October 1, 2008; 136(4): 433 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.Y. Turco, K. Matsukawa, M. Czernik, V. Gasperi, N. Battista, L. Della Salda, P.A. Scapolo, P. Loi, M. Maccarrone, and G. Ptak High levels of anandamide, an endogenous cannabinoid, block the growth of sheep preimplantation embryos by inducing apoptosis and reversible arrest of cell proliferation Hum. Reprod., October 1, 2008; 23(10): 2331 - 2338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.H. Betts and P. Madan Permanent embryo arrest: molecular and cellular concepts Mol. Hum. Reprod., August 1, 2008; 14(8): 445 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M Brad, K. E M Hendricks, and P. J Hansen The block to apoptosis in bovine two-cell embryos involves inhibition of caspase-9 activation and caspase-mediated DNA damage Reproduction, December 1, 2007; 134(6): 789 - 797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Takenaka, T. Horiuchi, and R. Yanagimachi Effects of light on development of mammalian zygotes PNAS, September 4, 2007; 104(36): 14289 - 14293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Vandaele, B. Mateusen, D. G D Maes, A. de Kruif, and A. Van Soom Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity Reproduction, April 1, 2007; 133(4): 709 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. F. Mastromonaco, L. A. Favetta, L. C. Smith, F. Filion, and W. A. King The Influence of Nuclear Content on Developmental Competence of Gaur x Cattle Hybrid In Vitro Fertilized and Somatic Cell Nuclear Transfer Embryos Biol Reprod, March 1, 2007; 76(3): 514 - 523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rodriguez, C. Diez, S. Ikeda, L.J. Royo, J.N. Caamano, C. Alonso-Montes, F. Goyache, I. Alvarez, N. Facal, and E. Gomez Retinoids during the in vitro transition from bovine morula to blastocyst Hum. Reprod., August 1, 2006; 21(8): 2149 - 2157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-Z. Liu, L.-J. Yao, M.-X. Jiang, Z.-L. Lei, L.-S. Zhang, Y.-L. Zhang, Q.-Y. Sun, Y.-L. Zheng, X.-F. Song, and D.-Y. Chen Apoptosis in rabbit embryos produced by fertilization or nuclear transfer with fibroblasts and cumulus cells Reproduction, September 1, 2005; 130(3): 359 - 366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Zheng, R. D. Schramm, and K. E. Latham Developmental Regulation and In Vitro Culture Effects on Expression of DNA Repair and Cell Cycle Checkpoint Control Genes in Rhesus Monkey Oocytes and Embryos Biol Reprod, June 1, 2005; 72(6): 1359 - 1369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mateusen, A. Van Soom, D. G D Maes, I. Donnay, L. Duchateau, and A.-S. Lequarre Porcine embryo development and fragmentation and their relation to apoptotic markers: a cinematographic and confocal laser scanning microscopic study Reproduction, April 1, 2005; 129(4): 443 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Esfandiari, T. Falcone, A. Agarwal, M. Attaran, D. R. Nelson, and R. K. Sharma Protein Supplementation and the Incidence of Apoptosis and Oxidative Stress in Mouse Embryos Obstet. Gynecol., March 1, 2005; 105(3): 653 - 660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pfister-Genskow, C. Myers, L. A. Childs, J. C. Lacson, T. Patterson, J. M. Betthauser, P. J. Goueleke, R. W. Koppang, G. Lange, P. Fisher, et al. Identification of Differentially Expressed Genes in Individual Bovine Preimplantation Embryos Produced by Nuclear Transfer: Improper Reprogramming of Genes Required for Development Biol Reprod, March 1, 2005; 72(3): 546 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jurisicova and B. M Acton Deadly decisions: the role of genes regulating programmed cell death in human preimplantation embryo development Reproduction, September 1, 2004; 128(3): 281 - 291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.A. Favetta, C. Robert, E.J. St.John, D.H. Betts, and W.A. King p66shc, but not p53, is involved in early arrest of in vitro-produced bovine embryos Mol. Hum. Reprod., June 1, 2004; 10(6): 383 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Beker-van Woudenberg, H. T.A. van Tol, B. A.J. Roelen, B. Colenbrander, and M. M. Bevers Estradiol and Its Membrane-Impermeable Conjugate (Estradiol-Bovine Serum Albumin) During In Vitro Maturation of Bovine Oocytes: Effects on Nuclear and Cytoplasmic Maturation, Cytoskeleton, and Embryo Quality Biol Reprod, May 1, 2004; 70(5): 1465 - 1474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Kidson, F J Rubio-Pomar, A Van Knegsel, H T A Van Tol, W Hazeleger, D W B Ducro-Steverink, B Colenbrander, S J Dieleman, and M M Bevers Quality of porcine blastocysts produced in vitro in the presence or absence of GH Reproduction, February 1, 2004; 127(2): 165 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M. Knijn, J. O. Gjorret, P. L.A.M. Vos, P. J.M. Hendriksen, B. C. van der Weijden, P. Maddox-Hyttel, and S. J. Dieleman Consequences of In Vivo Development and Subsequent Culture on Apoptosis, Cell Number, and Blastocyst Formation in Bovine Embryos Biol Reprod, October 1, 2003; 69(4): 1371 - 1378. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
