| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Embryo |
Department of Anatomy and Reproductive Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822
Several researchers have suggested recently that the embryonic-abembryonic (Em-Ab) axis of the mouse blastocyst is orthogonal to the first cleavage plane of the two-cell embryo. To determine the universality of this relationship, we used embryos of two different genotypes, F1 (C57BL/6 x DBA/2) and CD-1. The position of the first cleavage plane in the early blastocyst was determined by labeling a blastomere with the fluorescent lineage tracer DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) at the two-cell stage. Approximately one quarter of the blastocysts from both genotypes possessed an Em-Ab axis that respected the orthogonal relationship with the first cleavage plane. However, the remainder of the blastocysts deviated from the orthogonal relationship. This result indicates that the orthogonal orientation of the Em-Ab axis to the first cleavage plane is not a universal phenomenon. We also tested whether the angular relationship between the Em-Ab axis and first cleavage plane influences postimplantation embryo development. We sorted the blastocysts that had the Em-Ab axis orthogonal to the first cleavage plane from the ones that did not. These two types of blastocysts were transferred separately into surrogates, and fetal development was examined in late gestation. The results revealed that both types of blastocysts produced normal fetuses at a similar frequency. Thus, the relationship of the blastocyst axis to the first cleavage plane does not significantly influence later development.
2 Correspondence: Vernadeth B. Alarcón, Department of Anatomy and Reproductive Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, 1960 East-West Rd., Honolulu, HI 96822. FAX: 808 956 7316; vernadet{at}hawaii.edu
This article has been cited by other articles:
|
|
 |
|
  H. Honda, N. Motosugi, T. Nagai, M. Tanemura, and T. Hiiragi Computer simulation of emerging asymmetry in the mouse blastocyst Development, April 15, 2008; 135(8): 1407 - 1414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bischoff, D.-E. Parfitt, and M. Zernicka-Goetz Formation of the embryonic-abembryonic axis of the mouse blastocyst: relationships between orientation of early cleavage divisions and pattern of symmetric/asymmetric divisions Development, March 1, 2008; 135(5): 953 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-E. Dietrich and T. Hiiragi Stochastic patterning in the mouse pre-implantation embryo Development, December 1, 2007; 134(23): 4219 - 4231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kurotaki, K. Hatta, K. Nakao, Y.-i. Nabeshima, and T. Fujimori Blastocyst Axis Is Specified Independently of Early Cell Lineage But Aligns with the ZP Shape Science, May 4, 2007; 316(5825): 719 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wakayama, T. Hikichi, R. Suetsugu, Y. Sakaide, H.-T. Bui, E. Mizutani, and T. Wakayama Efficient Establishment of Mouse Embryonic Stem Cell Lines from Single Blastomeres and Polar Bodies Stem Cells, April 1, 2007; 25(4): 986 - 993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.L. Gardner The axis of polarity of the mouse blastocyst is specified before blastulation and independently of the zona pellucida Hum. Reprod., March 1, 2007; 22(3): 798 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Waksmundzka, A. Wisniewska, and M. Maleszewski Allocation of Cells in Mouse Blastocyst Is Not Determined by the Order of Cleavage of the First Two Blastomeres Biol Reprod, October 1, 2006; 75(4): 582 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Deb, M. Sivaguru, H. Y. Yong, and R. M. Roberts Cdx2 Gene Expression and Trophectoderm Lineage Specification in Mouse Embryos Science, February 17, 2006; 311(5763): 992 - 996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.L. Gardner and T.J. Davies An investigation of the origin and significance of bilateral symmetry of the pronuclear zygote in the mouse Hum. Reprod., February 1, 2006; 21(2): 492 - 502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Motosugi, T. Bauer, Z. Polanski, D. Solter, and T. Hiiragi Polarity of the mouse embryo is established at blastocyst and is not prepatterned Genes & Dev., May 1, 2005; 19(9): 1081 - 1092. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
