| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reproductive Technology |
RIKEN Bioresource Center,3 Tsukuba, Ibaraki 305-0074, Japan
CREST,4 JST, Saitama 332-0012, Japan
Department of Veterinary Science,5 National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan
Gene Research Center,6 Tokyo Institute of Technology, Yokohama 226-8501, Japan
Although it is widely assumed that the cell type and genotype of the donor cell affect the efficiency of somatic cell cloning, little systematic analysis has been done to verify this assumption. The present study was undertaken to examine whether donor cell type, donor genotype, or a combination thereof increased the efficiency of mouse cloning. Initially we assessed the developmental ability of embryos that were cloned from cumulus or immature Sertoli cells with six different genotypes (i.e., 2 x 6 factorial). Significantly better cleavage rates were obtained with cumulus cells than with Sertoli cells (P < 0.005, two-way ANOVA), which probably was due to the superior cell-cycle synchrony of cumulus cells at G0/G1. After embryo transfer, there was a significant effect of cell type on the birth rate, with Sertoli cells giving the better result (P < 0.005). Furthermore, there was a significant interaction (P < 0.05) between the cell type and genotype, which indicates that cloning efficiency is determined by a combination of these two factors. The highest mean birth rate (10.8 ± 2.1%) was obtained with (B6 x 129)F1 Sertoli cells. In the second series of experiments, we examined whether the developmental ability of clones with the wild-type genotype (JF1) was improved when combined with the 129 genotype. Normal pups were cloned from cumulus and immature Sertoli cells of the (129 x JF1)F1 and (JF1 x 129)F1 genotypes, whereas no pups were born from cells with the (B6 x JF1)F1 genotype. The present study clearly demonstrates that the efficiency of somatic cell cloning, and in particular fetal survival after embryo transfer, may be improved significantly by choosing the appropriate combinations of cell type and genotype.
2 Correspondence: A. Ogura, RIKEN Bioresource Center, 3-1-1, Koyadai, Tsukuba, Ibaraki 305-0074, Japan. FAX: 81 29 836 9172; ogura{at}rtc.riken.go.jp
This article has been cited by other articles:
|
|
 |
|
  S. Wakayama, H. Ohta, T. Hikichi, E. Mizutani, T. Iwaki, O. Kanagawa, and T. Wakayama Production of healthy cloned mice from bodies frozen at -20{degrees}C for 16 years PNAS, November 11, 2008; 105(45): 17318 - 17322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li and P. Mombaerts Nuclear Transfer-Mediated Rescue of the Nuclear Genome of Nonviable Mouse Cells Frozen Without Cryoprotectant Biol Reprod, October 1, 2008; 79(4): 588 - 593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jincho, Y. Sotomaru, M. Kawahara, Y. Ono, H. Ogawa, Y. Obata, and T. Kono Identification of Genes Aberrantly Expressed in Mouse Embryonic Stem Cell-Cloned Blastocysts Biol Reprod, April 1, 2008; 78(4): 568 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hikichi, T. Kohda, S. Wakayama, F. Ishino, and T. Wakayama Nuclear Transfer Alters the DNA Methylation Status of Specific Genes in Fertilized and Parthenogenetically Activated Mouse Embryonic Stem Cells Stem Cells, March 1, 2008; 26(3): 783 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Inoue, S. Noda, N. Ogonuki, H. Miki, S. Inoue, K. Katayama, K. Mekada, H. Miyoshi, and A. Ogura Differential Developmental Ability of Embryos Cloned from Tissue-Specific Stem Cells Stem Cells, May 1, 2007; 25(5): 1279 - 1285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li, V. Greco, G. Guasch, E. Fuchs, and P. Mombaerts Mice cloned from skin cells PNAS, February 20, 2007; 104(8): 2738 - 2743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hikichi, S. Wakayama, E. Mizutani, Y. Takashima, S. Kishigami, N. Van Thuan, H. Ohta, H. Thuy Bui, S.-I. Nishikawa, and T. Wakayama Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer Stem Cells, January 1, 2007; 25(1): 46 - 53. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ono and T. Kono Irreversible Barrier to the Reprogramming of Donor Cells in Cloning with Mouse Embryos and Embryonic Stem Cells Biol Reprod, August 1, 2006; 75(2): 210 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Inoue, N. Ogonuki, H. Miki, M. Hirose, S. Noda, J.-M. Kim, F. Aoki, H. Miyoshi, and A. Ogura Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer J. Cell Sci., May 15, 2006; 119(10): 1985 - 1991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Van Thuan, S. Wakayama, S. Kishigami, H. Ohta, T. Hikichi, E. Mizutani, H.-T. Bui, and T. Wakayama Injection of Somatic Cell Cytoplasm into Oocytes Before Intracytoplasmic Sperm Injection Impairs Full-Term Development and Increases Placental Weight in Mice Biol Reprod, May 1, 2006; 74(5): 865 - 873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ohta and T. Wakayama Generation of Normal Progeny by Intracytoplasmic Sperm Injection Following Grafting of Testicular Tissue from Cloned Mice That Died Postnatally Biol Reprod, September 1, 2005; 73(3): 390 - 395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wakayama, H. Ohta, S. Kishigami, N. Van Thuan, T. Hikichi, E. Mizutani, M. Miyake, and T. Wakayama Establishment of Male and Female Nuclear Transfer Embryonic Stem Cell Lines from Different Mouse Strains and Tissues Biol Reprod, April 1, 2005; 72(4): 932 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wakayama, S. Kishigami, N. Van Thuan, H. Ohta, T. Hikichi, E. Mizutani, R. Yanagimachi, and T. Wakayama From The Cover: Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology PNAS, January 4, 2005; 102(1): 29 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Miki, K. Inoue, N. Ogonuki, K. Mochida, H. Nagashima, T. Baba, and A. Ogura Cytoplasmic Asters Are Required for Progression Past the First Cell Cycle in Cloned Mouse Embryos Biol Reprod, December 1, 2004; 71(6): 2022 - 2028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gao, E. Czirr, Y. G. Chung, Z. Han, and K. E. Latham Genetic Variation in Oocyte Phenotype Revealed Through Parthenogenesis and Cloning: Correlation with Differences in Pronuclear Epigenetic Modification Biol Reprod, April 1, 2004; 70(4): 1162 - 1170. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
