HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 69/5/1488    most recent biolreprod.103.017038v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kaneko, H. Articles by Akita, T. Search for Related Content PubMed PubMed Citation Articles by Kaneko, H. Articles by Akita, T. Agricola Articles by Kaneko, H. Articles by Akita, T.

Maturation and Fertilization of Porcine Oocytes from Primordial Follicles by a Combination of Xenografting and In Vitro Culture¹

Genetic Diversity Department,³ National institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan Agriculture, Forestry and Fisheries Research Council,⁴ Ministry of Agriculture, Forestry and Fisheries, Kasumigaseki, Tokyo 100-8950, Japan Department of Research Planning and Coordination,⁵ National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0091, Japan

Our objective was to develop a method of endowing oocytes from porcine primordial follicles with full maturation and fertilizing ability as a model for ovarian xenografting of large mammals. Ovarian tissues from 20-day-old piglets, in which most of the follicles were primordial, were transplanted under the capsules of both kidneys of ovariectomized athymic mice. The host mice were treated with 5 IU of equine chorionic gonadotropin (eCG) for 10 days (eCG-10), 30 days (eCG-30), or 60 days (eCG-60) after detection of cornified epithelial cells in their vaginal smears. Cumulus-oocyte complexes, ovarian grafts, and blood samples were obtained 48 h after eCG treatment. Forty-five to 70 days after grafting, the host mice in all groups for the first time showed vaginal cornification, accompanied by the formation of a small number of antral follicles in the grafts. However, we recovered large numbers of full-sized oocytes only from mice in the eCG-60 group; the numbers of full-sized oocytes in the other groups were low. Peripheral levels of total inhibin were highest in the eCG-60 group; this supports our finding that the most enhanced growth of antral follicles occurred in this eCG-60 group. Of 573 oocytes obtained from the eCG-60 group, 98 (17%) were at the metaphase II stage after in vitro culture for maturation. Moreover, 55% of matured oocytes with the first polar body (n = 20) were fertilized in vitro. These results clearly demonstrate that fertilization of oocytes from porcine primordial follicles is achievable by a combination of xenografting and in vitro culture.

¹ H.K. and K.K. contributed equally to this work.

² Correspondence: Hiroyuki Kaneko, Genetic Diversity Department, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan. FAX: 81 29 838 7447; kaneko{at}nias.affrc.go.jp

This article has been cited by other articles:

				A Bonnet, R Dalbies-Tran, and M A Sirard Opportunities and challenges in applying genomics to the study of oogenesis and folliculogenesis in farm animals Reproduction, February 1, 2008; 135(2): 119 - 128. [Abstract] [Full Text] [PDF]

				H. Kaneko, K. Kikuchi, J. Noguchi, M. Ozawa, K. Ohnuma, N. Maedomari, and N. Kashiwazaki Effects of gonadotrophin treatments on meiotic and developmental competence of oocytes in porcine primordial follicles following xenografting to nude mice Reproduction, February 1, 2006; 131(2): 279 - 288. [Abstract] [Full Text] [PDF]