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BOR - Papers in Press, published online ahead of print July 30, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.019307
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BIOLOGY OF REPRODUCTION 69, 1697–1706 (2003)
DOI: 10.1095/biolreprod.103.019307
© 2003 by the Society for the Study of Reproduction, Inc.

Female Reproductive Tract

Isolation of Differentially Expressed Genes in Conceptuses and Endometrial Tissue of Sows in Early Gestation1

Maud Vallée3, Danièle Beaudry4, Claude Roberge4, J. Jacques Matte4, Richard Blouin3, and Marie-France Palin2,4

Département de Biologie,3 Université de Sherbrooke, Sherbrooke, Québec, Canada J1K 2R1 Dairy and Swine Research and Development Center,4 Agriculture and Agri-Food Canada, Lennoxville, Québec, Canada J1M 1Z3

The implantation period is a critical time for embryonic survival in pigs. During this period, numerous growth factors are secreted by the conceptuses and the uterine endometrium in order to establish pregnancy and to provide a proper environment for embryonic development. It is well known that the Chinese Meishan sows have a larger litter size when compared with occidental sows mainly because of a superior embryonic survival rate. As a further step toward understanding the mechanisms involved in embryonic survival, we used a suppression subtractive hybridization technique to identify genes that were differentially expressed in Meishan-Landrace conceptuses and endometrial tissue at Day 15 of gestation when compared with conventional Landrace sows. Of the 1000 subtractive clones isolated from each library, 137 endometrial and 166 conceptus-enriched cDNAs were single-pass sequenced and examined by BLAST analysis for identification. Sixty-two percent of the clones found in the endometrial library and 78% of the clones found in the conceptus library showed homology with known genes. Among these genes, the 20 most relevant to embryonic survival based on the available literature were validated through real-time reverse-transcriptase polymerase chain reaction (RT-PCR) analysis. Our results show that suppression subtractive hybridization is a powerful method applicable in identifying putative candidate genes that might be used for selection of high litter-size breeds.

1 This work was supported by Genetiporc Inc. (St-Bernard, QC, Canada) and Agriculture and Agri-Food Canada. Lennoxville Dairy and Swine R&D Centre contribution 799. M.V. is supported by a FCAR fellowship.

2 Correspondence: Marie-France Palin, Agriculture and Agri-Food Canada, P.O. Box 90, 2000 Route 108 East, Lennoxville, Quebec, J1M 1Z3 Canada. FAX: 819 564 5507; palinmf{at}agr.gc.ca






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Copyright © 2003 by the Society for the Study of Reproduction.