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BOR - Papers in Press, published online ahead of print July 30, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.017350
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BIOLOGY OF REPRODUCTION 69, 1801–1807 (2003)
DOI: 10.1095/biolreprod.103.017350
© 2003 by the Society for the Study of Reproduction, Inc.

Gamete Biology

Tyrosine Phosphorylation of HSP-90 During Mammalian Sperm Capacitation1

Heath Ecroyd3, Russell C. Jones3, and R. John Aitken2,4

Reproductive Science Group,3 School of Environmental and Life Sciences, ARC Centre of Excellence for Biotechnology and Development,4 University of Newcastle, Callaghan, New South Wales 2308, Australia

The process of sperm capacitation is correlated with activation of a signal transduction pathway leading to protein tyrosine phosphorylation. Whereas phosphotyrosine expression is an essential prerequisite for fertilization, the proteins that are phosphorylated during capacitation have not yet been identified. In the present study, we observed that a major target of this signaling pathway is the molecular chaperone protein, heat shock protein (HSP)-86, a member of the HSP-90 family of HSPs. We used cross-immunoprecipitation experiments to confirm the tyrosine phosphorylation of HSP-86, a process that is not inhibited by the ansamycin antibiotic, geldanamycin. The general significance of these findings was confirmed by studies in which HSP-90 was also found to be tyrosine phosphorylated in human and rat spermatozoa when incubated under conditions that support capacitation. To our knowledge, these results represent the first report of a protein that undergoes tyrosine phosphorylation during mouse sperm capacitation and the first study implicating molecular chaperones in the processes by which mammalian spermatozoa gain the ability to fertilize the oocyte.

1 Supported by an Australian Research Council project grant (A0099180338).

2 Correspondence: R. John Aitken, Reproductive Science Group, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW 2308, Australia. FAX: 61 2 49 21 6308; jaitken{at}mail.newcastle.edu.au




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