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BOR - Papers in Press, published online ahead of print August 6, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.017384
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BIOLOGY OF REPRODUCTION 69, 1863–1871 (2003)
DOI: 10.1095/biolreprod.103.017384
© 2003 by the Society for the Study of Reproduction, Inc.

Embryo

Identification of a Biological Activity That Supports Maintenance and Proliferation of Pluripotent Cells from the Primitive Ectoderm of the Mouse1

Joy Rathjen, Jennifer M. Washington3, Michael D. Bettess4, and Peter D. Rathjen2

School of Molecular and Biomedical Sciences, and Australian Research Council Special Research Centre for Molecular Genetics of Development, University of Adelaide, Adelaide, South Australia 5005, Australia

Pluripotent cell development in the mammalian embryo results in the sequential formation of several developmentally distinct populations, inner cell mass, primitive ectoderm, and the primordial germ lineage. Factors within medium conditioned by HepG2 cells (MEDII) have been implicated in the formation and maintenance of primitive ectoderm from inner cell mass cells both in vitro and in vivo. Here we demonstrate that MEDII, but not LIF, is able to support the maintenance and proliferation in culture of pluripotent cells derived from primitive ectoderm formed in vitro or during embryonic development. This distinguishes primitive ectoderm and inner cell mass (ICM) on the basis of cytokine responsiveness and validates the biological activity proposed for factors within MEDII in primitive ectoderm establishment and maintenance. Further, it potentially provides an alternative technology for the isolation of pluripotent cells from the mammalian embryo.

1 This work was supported by the Australian Research Council and Bresagen Pty. Ltd.

3 Current address: School of Agriculture and Wine, Waite Campus, University of Adelaide, Urrbrae, South Australia 5034 Australia

4 Current address: Institute Suisse de Recherche Expérimentale sur le Cancer (ISREC), Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland

2 Correspondence: FAX: 618 8303 4348; peter.rathjen{at}adelaide.edu.au




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A. M. Wobus and K. R. Boheler
Embryonic Stem Cells: Prospects for Developmental Biology and Cell Therapy
Physiol Rev, April 1, 2005; 85(2): 635 - 678.
[Abstract] [Full Text] [PDF]


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Copyright © 2003 by the Society for the Study of Reproduction.