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This Article Full Text Full Text (PDF) All Versions of this Article: 69/6/1890    most recent biolreprod.103.018945v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tani, T. Articles by Tsunoda, Y. Search for Related Content PubMed PubMed Citation Articles by Tani, T. Articles by Tsunoda, Y. Agricola Articles by Tani, T. Articles by Tsunoda, Y.

Reprogramming of Bovine Somatic Cell Nuclei Is Not Directly Regulated by Maturation Promoting Factor or Mitogen-Activated Protein Kinase Activity¹

Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631-8505, Japan

Cloned mammals with normal fertility have been produced by nuclear transfer. Thus, oocyte cytoplasm has the ability to convert differentiated somatic cell nuclei into a state that resembles the conditions that occur at fertilization (nuclear reprogramming). Despite the long-held assumption that reprogramming factors are present in mammalian oocytes, the molecular nature of these factors is not known. The present study demonstrates that the process of nuclear reprogramming is not directly regulated by maturation promoting factor or mitogen-activated protein kinase activity. The potential for nuclear-transferred oocytes to develop to the blastocyst stage was not different when somatic cells at the M phase were fused with oocytes activated with ionomycin and cycloheximide 1–5 h before (12%–22%) but was significantly decreased when oocytes were activated 6 h before (1%). Further molecular studies on the differences between oocytes with and without reprogramming potential are required and will be useful for the identification of reprogramming factors.

¹ This work was supported by grants from the Ministry of Education, Science, and Culture (13308054, 14034259, 15039233) and the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN).

² Correspondence: Yukio Tsunoda, Laboratory of Animal Reproduction, College of Agriculture, Kinki University, 3327-204, Nakamachi, Nara, 631-8505 Japan. FAX: 81 742 43 1155; tsunoda{at}nara.kindai.ac.jp

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