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This Article Full Text Full Text (PDF) All Versions of this Article: 69/6/1945    most recent biolreprod.103.018150v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klemcke, H.G. Articles by Christenson, R.K. Search for Related Content PubMed PubMed Citation Articles by Klemcke, H.G. Articles by Christenson, R.K. Agricola Articles by Klemcke, H.G. Articles by Christenson, R.K.

11ß-Hydroxysteroid Dehydrogenase and Glucocorticoid Receptor Messenger RNA Expression in Porcine Placentae: Effects of Stage of Gestation, Breed, and Uterine Environment¹

USDA-ARS,⁴ Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933 The Lawson Research Institute,⁵ St. Joseph's Hospital, Departments of Obstetrics and Gynecology and Physiology, University of Western Ontario, London, Ontario, Canada N6A 4V2

Glucocorticoids are known to influence many aspects of prenatal development. Three important regulators of glucocorticoid actions at the cellular level are the enzymes 11ß-hydroxysteroid dehydrogenase type 1 (11ßHSD-1), 11ß-hydroxysteroid dehydrogenase type 2 (11ßHSD-2), and glucocorticoid receptors (GR). The present study was conducted to determine the presence of these regulators in porcine placentae during early gestation (Days 24–40; term = 114 days) and to examine the influence of breed and uterine environment. Three pig models differing in uterine environment as reflected by embryonic survival from Days 24 to 40 were used: intact white cross-bred gilts (WC-INT); white cross-bred gilts that had been unilaterally hysterectomized-ovariectomized before puberty (WC-UHO); and intact Meishan gilts (ME). Porcine-specific partial cDNAs for 11ßHSD-1 and 11ßHSD-2 and a cRNA for GR were developed and used to produce ³²P-labeled probes for Northern blot analyses. The 11ßHSD dehydrogenase activity was measured in vitro at saturating concentrations of substrate and coenzyme. At Day 24 of gestation, 11ßHSD-2 mRNA, dehydrogenase activity, and GR mRNA were present, but 11ßHSD-1 mRNA was absent. All three mRNAs and dehydrogenase activity increased (P < 0.01) by Day 40. On Day 30, placental 11ßHSD-2 mRNA was decreased (P = 0.03) by 47% in WC-UHO versus WC-INT. Placental 11ßHSD dehydrogenase activity was 2-fold greater (P < 0.01) in ME versus WC-INT on Day 24 of gestation. These results demonstrate, to our knowledge for the first time, the presence of 11ßHSD-1, 11ßHSD-2, and GR mRNA as well as 11ßHSD dehydrogenase activity in the porcine placenta during early pregnancy. Moreover, a role for glucocorticoids in porcine embryonic development is suggested.

¹ Mention of trade names is necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.

² Correspondence: H.G. Klemcke, U.S. Army Institute of Surgical Research, 3400 Rawley E. Chambers Ave., Fort Sam Houston, TX 78234. harold.klemcke{at}cen.amedd.army.mil

³ Current address: CuraGen Corporation, 16 Commercial Street, Branford, CT 06405

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